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Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354)


See all RAB8A antibodies
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Tested Data
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in ELISA
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in ELISA
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in WB
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in WB
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in WB
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in WB
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in FC
Mouse Anti-RAB8A Recombinant Antibody (CBT2692) (V2LY-0625-LY354) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2692
Antibody Isotype
IgG2b
Application
FC

Basic Information

Immunogen
Purified recombinant fragment of human Rab8 (AA: 68-207) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
RAB8A, Member RAS Oncogene Family
Entrez Gene ID
4218
UniProt ID
P61006
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297).
Regulates the compacted morphology of the Golgi (PubMed:26209634).
Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656).
Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed:21844891, PubMed:30398148).
Together with MICALL2, may also regulate adherens junction assembly (By similarity).
May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity).
Involved in autophagy (PubMed:27103069).
Biological Process
Biological Process autophagyIEA:UniProtKB-KW
Biological Process axonogenesisISS:UniProtKB
Biological Process cellular response to insulin stimulusISS:UniProtKB
Biological Process cilium assemblyManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process endocytic recyclingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process Golgi organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process Golgi vesicle fusion to target membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process neurotransmitter receptor transport to postsynaptic membraneManual Assertion Based On ExperimentIBA:GO_Central
Biological Process neurotransmitter receptor transport, endosome to postsynaptic membraneIEA:Ensembl
Biological Process protein localization to ciliumISS:UniProtKB
Biological Process protein localization to plasma membraneISS:UniProtKB
Biological Process protein secretionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of autophagyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of exocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of long-term neuronal synaptic plasticityIEA:Ensembl
Biological Process regulation of protein transportIEA:Ensembl
Biological Process vesicle docking involved in exocytosisManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process vesicle-mediated transport in synapseIEA:Ensembl
Cellular Location
Cell membrane
Golgi apparatus
Recycling endosome membrane
Cell projection, cilium
Cytoplasmic vesicle, phagosome
Cytoplasmic vesicle, phagosome membrane
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Cytoplasm, cytoskeleton, cilium basal body
Midbody
Cytoplasm
Cytoplasm, cytoskeleton, cilium axoneme
Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803).
In the GDP-bound form, present in the perinuclear region (PubMed:12221131).
Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131).
Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297).
Localizes to tubular recycling endosome (PubMed:19864458).
Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).
Non-phosphorylated RAB8A predominantly localizes to the cytoplasm whereas phosphorylated RAB8A localizes to the membrane (PubMed:26824392, PubMed:29125462, PubMed:30398148).
PTM
Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
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For research use only. Not intended for any clinical use.

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