Mouse Anti-RAB8A Recombinant Antibody (4/Rab8) (CBMAB-R0941-CN)

RAB8A, Member RAS Oncogene Family

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008]

RAB8A, Member RAS Oncogene Family; Mel Transforming Oncogene (Derived From Cell Line NK14); Oncogene C-Mel; RAB8; MEL; Mel Transforming Oncogene (Derived From Cell Line NK14)- RAB8 Homolog; Mel Transforming Oncogene (RAB8 Homolog); Ras-Associated Protein RAB8; Ras-Related Protein Rab-8A;

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (PubMed:20890297).
Regulates the compacted morphology of the Golgi (PubMed:26209634).
Together with MYO5B and RAB11A participates in epithelial cell polarization (PubMed:21282656).
Also involved in membrane trafficking to the cilium and ciliogenesis (PubMed:21844891, PubMed:30398148).
Together with MICALL2, may also regulate adherens junction assembly (By similarity).
May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity).
Involved in autophagy (PubMed:27103069).

Biological Process autophagyIEA:UniProtKB-KW
Biological Process axonogenesisISS:UniProtKB
Biological Process cellular response to insulin stimulusISS:UniProtKB
Biological Process cilium assemblyManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process endocytic recyclingManual Assertion Based On ExperimentIBA:GO_Central
Biological Process Golgi organizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process Golgi vesicle fusion to target membraneManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process neurotransmitter receptor transport to postsynaptic membraneManual Assertion Based On ExperimentIBA:GO_Central
Biological Process neurotransmitter receptor transport, endosome to postsynaptic membraneIEA:Ensembl
Biological Process protein localization to ciliumISS:UniProtKB
Biological Process protein localization to plasma membraneISS:UniProtKB
Biological Process protein secretionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of autophagyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of exocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of long-term neuronal synaptic plasticityIEA:Ensembl
Biological Process regulation of protein transportIEA:Ensembl
Biological Process vesicle docking involved in exocytosisManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process vesicle-mediated transport in synapseIEA:Ensembl

Cell membrane
Golgi apparatus
Recycling endosome membrane
Cell projection, cilium
Cytoplasmic vesicle, phagosome
Cytoplasmic vesicle, phagosome membrane
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Cytoplasm, cytoskeleton, cilium basal body
Midbody
Cytoplasm
Cytoplasm, cytoskeleton, cilium axoneme
Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803).
In the GDP-bound form, present in the perinuclear region (PubMed:12221131).
Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form (PubMed:12221131).
Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297).
Localizes to tubular recycling endosome (PubMed:19864458).
Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).
Non-phosphorylated RAB8A predominantly localizes to the cytoplasm whereas phosphorylated RAB8A localizes to the membrane (PubMed:26824392, PubMed:29125462, PubMed:30398148).

Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.