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Mouse Anti-RAD21 Recombinant Antibody (CBYY-C2093) (CBMAB-C3531-YY)

This product is mouse antibody that recognizes RAD21. The antibody CBYY-C2093 can be used for immunoassay techniques such as: WB
See all RAD21 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBYY-C2093
Antibody Isotype
IgG1, κ
Application
WB

Basic Information

Immunogen
An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21)
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
1 mg/mL
Preservative
PBS, pH 7.2
Concentration
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
RAD21 cohesin complex component
Entrez Gene ID
Human5885
Mouse19357
UniProt ID
HumanO60216
MouseQ61550
Alternative Names
Selectin P Ligand; PSGL-1; Cutaneous Lymphocyte-Associated Associated Antigen; P-Selectin Glycoprotein Ligand 1; CD162 Antigen; CD162; PSGL1; CLA;
Function
Double-strand-break repair protein rad21 homolog
As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732).
The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136).
In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity).
May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569).
May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).
64-kDa C-terminal product
May promote apoptosis.
Biological Process
Biological Process apoptotic processIEA:UniProtKB-KW
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process DNA recombinationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process double-strand break repairManual Assertion Based On ExperimentTAS:ProtInc
Biological Process establishment of meiotic sister chromatid cohesion1 PublicationIC:ComplexPortal
Biological Process negative regulation of G2/M transition of mitotic cell cycleIEA:Ensembl
Biological Process negative regulation of mitotic metaphase/anaphase transitionIEA:Ensembl
Biological Process positive regulation of sister chromatid cohesionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein localization to chromatinManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process reciprocal meiotic recombinationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process replication-born double-strand break repair via sister chromatid exchangeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process sister chromatid cohesionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Double-strand-break repair protein rad21 homolog
Nucleus
Nucleus matrix
Chromosome
Chromosome, centromere
Cytoplasm, cytoskeleton, spindle pole
Associates with chromatin (PubMed:11590136, PubMed:11073952).
Before prophase, scattered along chromosome arms (PubMed:11073952).
During prophase and prometaphase, most cohesins dissociate from the arms of condensing chromosome, possibly through PLK1-mediated phosphorylation (PubMed:11931760).
A small amount of cohesin remains in centromeric regions and is removed from chromosomes only at the onset of anaphase. At anaphase, cleavage by separase/ESPL1 leads to the dissociation of cohesin from chromosomes and chromosome separation (PubMed:11073952, PubMed:11509732).
64-kDa C-terminal product
Cytoplasm, cytosol
Nucleus
Involvement in disease
Cornelia de Lange syndrome 4 with or without midline brain defects (CDLS4):
A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. It is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
Mungan syndrome (MGS):
An autosomal recessive disease characterized by visceral neuromyopathy, intestinal dysmotility and chronic intestinal pseudoobstruction, megaduodenum, long-segment Barrett esophagus, and a variety of cardiac valve or septal defects such as membranous ventricular septal defect, pulmonary and tricuspid valve regurgitation.
PTM
Cleaved by separase/ESPL1 at the onset of anaphase; this cleavage is required for sister chromatid separation and cytokinesis (PubMed:11509732).
Cleaved by caspase-3/CASP3 or caspase-7/CASP7 at the beginning of apoptosis (PubMed:12417729, PubMed:11875078).
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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