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Rabbit Anti-RAD21 Recombinant Antibody (D5Y8S) (CBMAB-R1056-CN)

This product is a Rabbit antibody that recognizes RAD21. The antibody D5Y8S can be used for immunoassay techniques such as: WB, IP.
See all RAD21 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey, Chicken, Cattle, Dog
Clone
D5Y8S
Application
WB, IP

Basic Information

Specificity
Human, Mouse, Rat, Monkey, Chicken, Cattle, Dog
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Target

Full Name
RAD21 cohesin complex component
Introduction
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human5885
Mouse19357
Rat314949
Chicken420286
Cattle540966
Dog482021
Monkey700633
UniProt ID
HumanO60216
MouseQ61550
RatQ4KLH7
ChickenA0A1D5PM87
CattleQ3SWX9
DogE2QRU9
MonkeyH9EMG5
Alternative Names
RAD21 Cohesin Complex Component; Sister Chromatid Cohesion 1; Nuclear Matrix Protein 1; SCC1 Homolog; Kleisin; NXP-1; HHR21; HR21; NXP1; Protein Involved In DNA Double-Strand Break Repair;
Function
Double-strand-break repair protein rad21 homolog
As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732).
The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136).
In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity).
May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569).
May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).
64-kDa C-terminal product
May promote apoptosis.
Biological Process
Biological Process apoptotic processIEA:UniProtKB-KW
Biological Process cell divisionIEA:UniProtKB-KW
Biological Process DNA recombinationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process double-strand break repairManual Assertion Based On ExperimentTAS:ProtInc
Biological Process establishment of meiotic sister chromatid cohesion1 PublicationIC:ComplexPortal
Biological Process negative regulation of G2/M transition of mitotic cell cycleIEA:Ensembl
Biological Process negative regulation of mitotic metaphase/anaphase transitionIEA:Ensembl
Biological Process positive regulation of sister chromatid cohesionManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein localization to chromatinManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process reciprocal meiotic recombinationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process replication-born double-strand break repair via sister chromatid exchangeManual Assertion Based On ExperimentIBA:GO_Central
Biological Process sister chromatid cohesionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Double-strand-break repair protein rad21 homolog
Nucleus
Nucleus matrix
Chromosome
Chromosome, centromere
Cytoplasm, cytoskeleton, spindle pole
Associates with chromatin (PubMed:11590136, PubMed:11073952).
Before prophase, scattered along chromosome arms (PubMed:11073952).
During prophase and prometaphase, most cohesins dissociate from the arms of condensing chromosome, possibly through PLK1-mediated phosphorylation (PubMed:11931760).
A small amount of cohesin remains in centromeric regions and is removed from chromosomes only at the onset of anaphase. At anaphase, cleavage by separase/ESPL1 leads to the dissociation of cohesin from chromosomes and chromosome separation (PubMed:11073952, PubMed:11509732).
64-kDa C-terminal product
Cytoplasm, cytosol
Nucleus
Involvement in disease
Cornelia de Lange syndrome 4 with or without midline brain defects (CDLS4):
A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. It is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
Mungan syndrome (MGS):
An autosomal recessive disease characterized by visceral neuromyopathy, intestinal dysmotility and chronic intestinal pseudoobstruction, megaduodenum, long-segment Barrett esophagus, and a variety of cardiac valve or septal defects such as membranous ventricular septal defect, pulmonary and tricuspid valve regurgitation.
PTM
Cleaved by separase/ESPL1 at the onset of anaphase; this cleavage is required for sister chromatid separation and cytokinesis (PubMed:11509732).
Cleaved by caspase-3/CASP3 or caspase-7/CASP7 at the beginning of apoptosis (PubMed:12417729, PubMed:11875078).
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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