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Rabbit Anti-SMURF2 Recombinant Antibody (CBXS-5701) (CBMAB-S0569-CQ)

This product is a rabbit antibody that recognizes SMURF2. The antibody CBXS-5701 can be used for immunoassay techniques such as: ICC, IF, WB.
See all SMURF2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBXS-5701
Antibody Isotype
IgG
Application
ICC, IF, WB

Basic Information

Immunogen
Protein corresponding to Human SMURF2 (aa 151-242)
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.09% sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
SMAD Specific E3 Ubiquitin Protein Ligase 2
Introduction
SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and Innate Immune System. Gene Ontology (GO) annotations related to this gene include identical protein binding and ubiquitin-protein transferase activity. An important paralog of this gene is SMURF1.
Entrez Gene ID
64750
UniProt ID
Q9HAU4
Alternative Names
SMAD Specific E3 Ubiquitin Protein Ligase 2; HECT-Type E3 Ubiquitin Transferase SMURF2; SMAD Ubiquitination Regulatory Factor 2; HSMURF2; SMAD-Specific E3 Ubiquitin-Protein Ligase 2; E3 Ubiquitin-Protein Ligase SMURF2; E3 Ubiquitin Ligase SMURF2; EC 2.3.2.26; EC 6.3.2;
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11016919).
Interacts with SMAD7 to trigger SMAD7-mediated transforming growth factor beta/TGF-beta receptor ubiquitin-dependent degradation, thereby down-regulating TGF-beta signaling (PubMed:11163210, PubMed:12717440).
In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with AIMP1 (PubMed:18448069).
Also forms a stable complex with TGF-beta receptor-mediated phosphorylated SMAD1, SMAD2 and SMAD3, and targets SMAD1 and SMAD2 for ubiquitination and proteasome-mediated degradation (PubMed:11016919, PubMed:11158580, PubMed:11389444).
SMAD2 may recruit substrates, such as SNON, for ubiquitin-dependent degradation (PubMed:11389444).
Negatively regulates TGFB1-induced epithelial-mesenchymal transition and myofibroblast differentiation (PubMed:30696809).
(Microbial infection) In case of filoviruses Ebola/EBOV and Marburg/MARV infection, the complex formed by viral matrix protein VP40 and SMURF2 facilitates virus budding.
Biological Process
Biological Process negative regulation of BMP signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Biological Process negative regulation of DNA-templated transcription1 PublicationNAS:UniProtKB
Biological Process negative regulation of transcription by RNA polymerase IITAS:Reactome
Biological Process negative regulation of transforming growth factor beta receptor signaling pathwayManual Assertion Based On ExperimentIDA:CACAO
Biological Process positive regulation of canonical Wnt signaling pathwayTAS:Reactome
Biological Process positive regulation of trophoblast cell migrationManual Assertion Based On ExperimentIDA:CACAO
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein polyubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of transforming growth factor beta receptor signaling pathway1 PublicationNAS:UniProtKB
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process ubiquitin-dependent SMAD protein catabolic processManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process Wnt signaling pathway, planar cell polarity pathwayTAS:Reactome
Cellular Location
Nucleus
Cytoplasm
Cell membrane
Membrane raft
Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
PTM
Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1 (PubMed:19343052, PubMed:30696809).
Ubiquitinated by the SCF(FBXL15) complex and TTC3, leading to its degradation by the proteasome (PubMed:21572392, PubMed:30696809).
'Lys-48'-linked polyubiquitination mediated by TRAF4 at Lys-119 leads to SMURF2 proteasomal degradation (PubMed:31076633).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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