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Mouse Anti-TET3 Recombinant Antibody (CBYJT-2581) (CBMAB-T1751-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJT-2581
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 241-568 of human TET3 (NP_659430) produced in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 241-568

Target

Full Name
Tet Methylcytosine Dioxygenase 3
Introduction
Members of the ten-eleven translocation (TET) gene family, including TET3, function in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).
Entrez Gene ID
200424
UniProt ID
O43151
Alternative Names
Tet Methylcytosine Dioxygenase 3; Tet Oncogene Family Member 3; EC 1.14.11.n2; Probable Methylcytosine Dioxygenase TET3; Putative Methylcytosine Dioxygenase; Methylcytosine Dioxygenase TET3; HCG_40738; KIAA0401
Function
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization (PubMed:31928709).
Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity).
Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes (PubMed:23217707).
In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity).
In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT (PubMed:23353889).
Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (PubMed:29276034).
Biological Process
Biological Process 5-methylcytosine catabolic processSource:InterPro
Biological Process chromatin organizationSource:UniProtKB-KW
Biological Process DNA demethylationSource:UniProtKB
Biological Process DNA demethylation of male pronucleusSource:UniProtKB
Biological Process histone H3-K4 trimethylationSource:UniProtKB1 Publication
Biological Process oxidative demethylationSource:GO_Central1 Publication
Biological Process positive regulation of transcription by RNA polymerase IISource:UniProtKB1 Publication
Biological Process protein O-linked glycosylationSource:UniProtKB1 Publication
Cellular Location
Nucleus
Cytoplasm
Chromosome
At the zygotic stage, localizes in the male pronucleus, while it localizes to the cytoplasm at other preimplantation stages. Binds to the promoter of target genes, close to the transcription start site.
Involvement in disease
Beck-Fahrner syndrome (BEFAHRS):
A developmental disorder characterized by mild to severe intellectual disability, global developmental delay, hypotonia, autistic traits, movement disorders, growth abnormalities including overgrowth or poor growth, and facial dysmorphism. Both autosomal dominant and autosomal recessive inheritance has been reported.
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For research use only. Not intended for any clinical use.

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