Mouse Anti-USP30 Recombinant Antibody (CBXU-093) (CBMAB-U0192-CQ)

Name: Mouse Anti-USP30 Recombinant Antibody (CBXU-093)
SKU: CBMAB-U0192-CQ
Rating: 5 (1 reviews)

See all USP30 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXU-093

Application

WB, IHC-P

Immunogen

Recombinant protein corresponding to amino acid sequence: IEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPVVPDYSSST

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

>95% as determined by SDS-PAGE analysis

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ubiquitin specific peptidase 30

Introduction

USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).

Entrez Gene ID

84749

UniProt ID

Q70CQ3

Alternative Names

USP30; ubiquitin specific peptidase 30; ubiquitin specific protease 30; ubiquitin carboxyl-terminal hydrolase 30; FLJ40511; MGC10702; ub-specific protease 30; ubiquitin thioesterase 30; deubiquitinating enzyme 30

Function

Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951).
Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling (PubMed:25621951).
Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291).
Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity).

Biological Process

Biological Process autophagy of mitochondrion Source:UniProtKB1 Publication
Biological Process mitochondrial fusion Source:UniProtKB
Biological Process negative regulation of mitophagy Source:Ensembl
Biological Process pexophagy Source:Reactome
Biological Process protein deubiquitination Source:UniProtKB1 Publication
Biological Process protein K11-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein K6-linked deubiquitination Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

Mitochondrion outer membrane

Topology

Mitochondrial intermembrane: 1-35
Helical: 36-56
Cytoplasmic: 57-517

PTM

Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-USP30 Recombinant Antibody (CBXU-093)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-USP30 Recombinant Antibody (CBXU-013) (CAT#: CBMAB-U0129-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry