Mouse Anti-CCT8 Recombinant Antibody (EG529) (CBMAB-EN602-LY)

Name: Mouse Anti-CCT8 Recombinant Antibody (EG529)
SKU: CBMAB-EN602-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCT8 Recombinant Antibody (EG529) (CBMAB-EN602-LY) in IP

Immunoprecipitation of cell lysates from H1703 cells transfected with Flag-tagged THOC3 using anti-CCT8, anti-CCT6A, anti-Flag, or IgG antibody, and immunoprecipitates were blotted with anti-Flag/CCT8/CCT6A antibodies. ...View More

Yu, T., Zhang, Q., Yu, S. K., Nie, F. Q., Zhang, M. L., Wang, Q., & Lu, K. H. (2023). THOC3 interacts with YBX1 to promote lung squamous cell carcinoma progression through PFKFB4 mRNA modification. Cell Death & Disease, 14(7), 475. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CCT8 Recombinant Antibody (EG529) (CBMAB-EN602-LY) in WB

Relative levels of target proteins in CuO-POTEF-Flag HGrC1 cells with POTEF expression. Cell lysates were western blotted with anti-Flag, anti-TCP-1α, anti-TCP-1θ, and anti-PLK1 antibodies. ...View More

Kasahara, Y., Osuka, S., Takasaki, N., Bayasula, Koya, Y., Nakanishi, N., ... & Kajiyama, H. (2021). Primate-specific POTE-actin gene could play a role in human folliculogenesis by controlling the proliferation of granulosa cells. Cell Death Discovery, 7(1), 186. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CCT8 Recombinant Antibody (EG529) (CBMAB-EN602-LY) in WB

Representative immunoblot of fractionated brain tissue. A = unfractionated brain, B = nuclear fraction, C = synaptosomal fraction, and D = mitochondrial fraction. Fractionation was validated by the enrichment of proteins known to reside in each compartment: HDAC2(nuclear protein), SynGAP(synaptic protein), and COX IV (mitochondrial protein). The images represent five separately processed immunoblots. ...View More

McClatchy, D. B., Martínez-Bartolomé, S., Gao, Y., Lavallée-Adam, M., & Yates III, J. R. (2020). Quantitative analysis of global protein stability rates in tissues. Scientific Reports, 10(1), 15983. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG529

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 2-29 at the N-terminus of TCP-1 θ of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Horse, Rat, Cattle, Dog, Pig, Sheep

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chaperonin Containing TCP1 Subunit 8

Introduction

CCT8 (Chaperonin Containing TCP1 Subunit 8) is a Protein Coding gene. Among its related pathways are Organelle biogenesis and maintenance and Innate Immune System. Gene Ontology (GO) annotations related to this gene include unfolded protein binding and ATPase activity, coupled. An important paralog of this gene is CCT8L2.

Entrez Gene ID

Human	10694
Mouse	12469

UniProt ID

Human	P50990
Mouse	P42932

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444).
The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444).
As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638).
The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Biological Process

Binding of sperm to zona pellucida Source: Ensembl
Neutrophil degranulation Source: Reactome
Pore complex assembly Source: Ensembl
Positive regulation of establishment of protein localization to telomere Source: BHF-UCL
Positive regulation of protein localization to Cajal body Source: BHF-UCL
Positive regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Protein folding Source: FlyBase
Protein stabilization Source: BHF-UCL
Toxin transport Source: Ensembl

Cellular Location

Centrosome; Cilium basal body; Cytoplasm

Liao, Q., Ren, Y., Yang, Y., Zhu, X., Zhi, Y., Zhang, Y., ... & Zhao, L. (2021). CCT8 recovers WTp53-suppressed cell cycle evolution and EMT to promote colorectal cancer progression. Oncogenesis, 10(12), 1-12.

Yang, X., Ren, H., Shao, Y., Sun, Y., Zhang, L., Li, H., ... & Fu, J. (2018). Chaperonin-containing T‑complex protein 1 subunit 8 promotes cell migration and invasion in human esophageal squamous cell carcinoma by regulating α-actin and β-tubulin expression. International journal of oncology, 52(6), 2021-2030.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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