NAT8

NAT8, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. NAT8 shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.

Full Name

N-Acetyltransferase 8 (Putative)

Function

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids (PubMed:20392701).

This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617).

May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241).

Biological Process

Amyloid-beta metabolic process Source: UniProtKB
Amyloid fibril formation Source: Reactome
Glutathione metabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Peptidyl-lysine N6-acetylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Response to xenobiotic stimulus Source: ProtInc

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane
Other locations
Endoplasmic reticulum-Golgi intermediate compartment membrane

Topology

Cytoplasmic: 1-42
Helical: 43-63
Lumenal: 64-227