Mouse Anti-NAT8 Recombinant Antibody (5A8) (CBMAB-N1225-WJ)

Name: Mouse Anti-NAT8 Recombinant Antibody (5A8)
SKU: CBMAB-N1225-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5A8

Application

WB, IHC-P, ICC

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

N-Acetyltransferase 8 (Putative)

Introduction

This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq, Sep 2008]

Entrez Gene ID

9027

UniProt ID

Q9UHE5

Alternative Names

N-Acetyltransferase 8 (Putative); N-Acetyltransferase 8 (GCN5-Related, Putative); Cysteinyl-Conjugate N-Acetyltransferase; Camello-Like Protein 1; N-Acetyltransferase 8; Acetyltransferase 2; ATase2; TSC501; CCNAT; CML1;

Function

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids (PubMed:20392701).

This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617).

May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241).

Biological Process

Amyloid-beta metabolic process Source: UniProtKB
Amyloid fibril formation Source: Reactome
Glutathione metabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Peptidyl-lysine N6-acetylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Response to xenobiotic stimulus Source: ProtInc

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane
Other locations
Endoplasmic reticulum-Golgi intermediate compartment membrane

Topology

Cytoplasmic: 1-42
Helical: 43-63
Lumenal: 64-227

Zhao, X., Yuan, H., Yang, H., Liu, Y., Xun, M., Li, X., ... & Wang, H. (2023). Erratum for Zhao et al.,“N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins”. Journal of Virology, 97(3), e00257-23.

Zhao, X., Yuan, H., Yang, H., Liu, Y., Xun, M., Li, X., ... & Wang, H. (2022). N-Acetyltransferase 8 promotes viral replication by increasing the stability of enterovirus 71 nonstructural proteins. Journal of Virology, 96(6), e00119-22.

Luo, S., Surapaneni, A., Zheng, Z., Rhee, E. P., Coresh, J., Hung, A. M., ... & Grams, M. E. (2021). NAT8 variants, N-acetylated amino acids, and progression of CKD. Clinical Journal of the American Society of Nephrology: CJASN, 16(1), 37.

Rigby, M. J., Ding, Y., Farrugia, M. A., Feig, M., Cortese, G. P., Mitchell, H., ... & Puglielli, L. (2020). The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway. Journal of neurochemistry, 154(4), 404-423.

Grácio, P. C., Gonçalves-Dias, C., Lopes-Coelho, F., Monteiro, E. C., Serpa, J., da Silva, C. L., & Pereira, S. A. (2019, February). Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy. In 2019 IEEE 6th Portuguese Meeting on Bioengineering (ENBENG) (pp. 1-5). IEEE.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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