Mouse Anti-NAT8 Recombinant Antibody (5A8) (CBMAB-N1225-WJ)

This product is a Mouse antibody that recognizes NAT8. The antibody 5A8 can be used for immunoassay techniques such as: WB, IHC-P, ICC.
See all NAT8 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

5A8

Antibody Isotype

IgG1

Application

WB, IHC-P, ICC

Basic Information

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

N-Acetyltransferase 8 (Putative)

Introduction

This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq, Sep 2008]

Entrez Gene ID

9027

UniProt ID

Q9UHE5

Alternative Names

N-Acetyltransferase 8 (Putative); N-Acetyltransferase 8 (GCN5-Related, Putative); Cysteinyl-Conjugate N-Acetyltransferase; Camello-Like Protein 1; N-Acetyltransferase 8; Acetyltransferase 2; ATase2; TSC501; CCNAT; CML1;

Function

Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids (PubMed:20392701).

This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617).

May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241).

Biological Process

Amyloid-beta metabolic process Source: UniProtKB
Amyloid fibril formation Source: Reactome
Glutathione metabolic process Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Peptidyl-lysine N6-acetylation Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Response to xenobiotic stimulus Source: ProtInc

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane
Other locations
Endoplasmic reticulum-Golgi intermediate compartment membrane

Topology

Cytoplasmic: 1-42
Helical: 43-63
Lumenal: 64-227

References

Zhao, X., Yuan, H., Yang, H., Liu, Y., Xun, M., Li, X., ... & Wang, H. (2023). Erratum for Zhao et al.,“N-Acetyltransferase 8 Promotes Viral Replication by Increasing the Stability of Enterovirus 71 Nonstructural Proteins”. Journal of Virology, 97(3), e00257-23.

Zhao, X., Yuan, H., Yang, H., Liu, Y., Xun, M., Li, X., ... & Wang, H. (2022). N-Acetyltransferase 8 promotes viral replication by increasing the stability of enterovirus 71 nonstructural proteins. Journal of Virology, 96(6), e00119-22.

Luo, S., Surapaneni, A., Zheng, Z., Rhee, E. P., Coresh, J., Hung, A. M., ... & Grams, M. E. (2021). NAT8 variants, N-acetylated amino acids, and progression of CKD. Clinical Journal of the American Society of Nephrology: CJASN, 16(1), 37.

Rigby, M. J., Ding, Y., Farrugia, M. A., Feig, M., Cortese, G. P., Mitchell, H., ... & Puglielli, L. (2020). The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway. Journal of neurochemistry, 154(4), 404-423.

Grácio, P. C., Gonçalves-Dias, C., Lopes-Coelho, F., Monteiro, E. C., Serpa, J., da Silva, C. L., & Pereira, S. A. (2019, February). Changes in N-acetyltransferase 8 in kidney tubular cell: Injury, recovery and mesenchymal stromal cell-based therapy. In 2019 IEEE 6th Portuguese Meeting on Bioengineering (ENBENG) (pp. 1-5). IEEE.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-NAT8 Recombinant Antibody (CBYJT-1486)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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