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Mouse Anti-AMBP Recombinant Antibody (CBYC-P012) (CBMAB-P0054-YC)


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Published Data
Mouse Anti-AMBP Recombinant Antibody (CBYC-P012) in WB
Mouse Anti-AMBP Recombinant Antibody (CBYC-P012) in WB
Western blot for vimentin and α-1-microglobulin/bikunin precursor (AMBP) in adipose tissue from controls (n = 7) and lean chronic kidney disease (CKD) patients (n = 13). β-actin was used as a loading control. Half of the CKD samples and all of the control samples were run on 1 gel and the other half of the CKD samples and the same control samples were run on a second gel (space restrictions prevented the loading of all samples on 1 gel). Blots of the control samples are shown only once for ease of understanding. AMBP is consistently higher in the CKD patients, whereas the lower molecular weight forms of vimentin are generally lower in the CKD patients as compared to the controls (visual assessment). ...View More
Gertow, J., Ng, C. Z., Branca, R. M. M., Werngren, O., Du, L., Kjellqvist, S., ... & Fisher, R. M. (2017). Altered protein composition of subcutaneous adipose tissue in chronic kidney disease. Kidney international reports, 2(6), 1208-1218.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-P012
Antibody Isotype
IgG1
Application
ELISA, IF, IHC, WB

Basic Information

Immunogen
Recombinant AMBP of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha-1-Microglobulin/Bikunin Precursor
Introduction
AMBP is a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
Entrez Gene ID
259
UniProt ID
P02760
Alternative Names
HCP; ITIL; ITILC; ITI; UTI; EDC1; HI30; A1M; IATIL
Function
Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.1 Publication
Trypstatin is a trypsin inhibitor.
Biological Process
Cell adhesion Source: UniProtKB
Female pregnancy Source: UniProtKB
Heme catabolic process Source: UniProtKB
Negative regulation of immune response Source: UniProtKB
Negative regulation of JNK cascade Source: UniProtKB
Protein catabolic process Source: Ensembl
Protein-chromophore linkage Source: UniProtKB-KW
Receptor-mediated endocytosis Source: Reactome
Viral process Source: UniProtKB-KW
Cellular Location
Secreted
PTM
The precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.
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For research use only. Not intended for any clinical use.

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