Mouse Anti-AMBP Recombinant Antibody (CB175A) (CBMAB-CA175LY)

Name: Mouse Anti-AMBP Recombinant Antibody (CB175A)
SKU: CBMAB-CA175LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CB175A

Application

IHC-P

Immunogen

Recombinant Human protein AMBP

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	1:30-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Alpha-1-Microglobulin/Bikunin Precursor

Introduction

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Entrez Gene ID

259

UniProt ID

P02760

Alternative Names

Alpha-1-Microglobulin/Bikunin Precursor; Complex-Forming Glycoprotein Heterogeneous In Charge; Inter-Alpha-Trypsin Inhibitor Light Chain; Growth-Inhibiting Protein 19; Uronic-Acid-Rich Protein; Protein HC; Trypstatin; Uristatin; Bikunin; ITIL;

Function

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.1 Publication
Trypstatin is a trypsin inhibitor.

Biological Process

Cell adhesion Source: UniProtKB
Female pregnancy Source: UniProtKB
Heme catabolic process Source: UniProtKB
Negative regulation of immune response Source: UniProtKB
Negative regulation of JNK cascade Source: UniProtKB
Protein catabolic process Source: Ensembl
Protein-chromophore linkage Source: UniProtKB-KW
Receptor-mediated endocytosis Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Secreted

PTM

The precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.

Frank, A. (2019). Expression and purification of Alpha-1-microglobulin-bikunin-precursor in E. coli.

Liu, H., Luo, D., Qiu, Y., Huang, Y., Chen, C., Song, X., ... & Zhou, Y. (2019). The Effect of AMBP SNPs, Their Haplotypes, and Gene–Environment Interactions on the Risk of Atherothrombotic Stroke Among the Chinese Population. Genetic testing and molecular biomarkers, 23(7), 487-494.

Han, J. M., & Kong, H. S. (2018). Identification of Single Nucleotide Polymorphisms in AMBP gene and their associations with economic traits in Korean cattle (Hanwoo). Journal of Animal Breeding and Genomics Vol, 2(3).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

AMBP Matched Antibody Pair (070) (CAT#: APMAB-070LY)

AMBP Matched Antibody Pair (071) (CAT#: APMAB-071LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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