Human Recombinant AMBP protein, His Tag (V2LY-0526-LY2353)

Name: Human Recombinant AMBP protein, His Tag
SKU: V2LY-0526-LY2353
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Yeast

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant AMBP protein, His Tag consist of Amino Acid: 206-348 and predicts a molecular mass of 17.4 kDa.

Molecule Mass

17.4 kDa

Sequence

Amino Acid: 206-348

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Alpha-1-Microglobulin/Bikunin Precursor

Function

Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.1 Publication
Trypstatin is a trypsin inhibitor.

Biological Process

Cell adhesion Source: UniProtKB
Female pregnancy Source: UniProtKB
Heme catabolic process Source: UniProtKB
Negative regulation of immune response Source: UniProtKB
Negative regulation of JNK cascade Source: UniProtKB
Protein catabolic process Source: Ensembl
Protein-chromophore linkage Source: UniProtKB-KW
Receptor-mediated endocytosis Source: Reactome
Viral process Source: UniProtKB-KW

Cellular Location

Secreted

PTM

The precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.

Frank, A. (2019). Expression and purification of Alpha-1-microglobulin-bikunin-precursor in E. coli.

Liu, H., Luo, D., Qiu, Y., Huang, Y., Chen, C., Song, X., ... & Zhou, Y. (2019). The Effect of AMBP SNPs, Their Haplotypes, and Gene–Environment Interactions on the Risk of Atherothrombotic Stroke Among the Chinese Population. Genetic testing and molecular biomarkers, 23(7), 487-494.

Han, J. M., & Kong, H. S. (2018). Identification of Single Nucleotide Polymorphisms in AMBP gene and their associations with economic traits in Korean cattle (Hanwoo). Journal of Animal Breeding and Genomics Vol, 2(3).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat AMBP ELISA Kit (CAT#: V2LY-0626-LY1397)

AMBP Matched Antibody Pair (070) (CAT#: APMAB-070LY)

AMBP Matched Antibody Pair (071) (CAT#: APMAB-071LY)

Human Recombinant AMBP protein, His Tag-1 (CAT#: V2LY-0526-LY2354)

Human Recombinant AMBP protein, Fc Tag (CAT#: V2LY-0526-LY2355)

Rat Recombinant AMBP protein, His Tag (CAT#: V2LY-0526-LY9183)

Antibody Pairs

AMBP Matched Antibody Pair (070) (CAT#: APMAB-070LY)

AMBP Matched Antibody Pair (071) (CAT#: APMAB-071LY)

Related Products

Mouse Anti-AMBP Recombinant Antibody (CB175A) (CAT#: CBMAB-CA175LY)

Rat Recombinant AMBP protein, His Tag (CAT#: V2LY-0526-LY9183)

Rat AMBP ELISA Kit (CAT#: V2LY-0626-LY1397)

Mouse Anti-AMBP Recombinant Antibody (4F4) (CAT#: CBMAB-A2508-YC)

AMBP Matched Antibody Pair (071) (CAT#: APMAB-071LY)

Mouse Anti-AMBP Recombinant Antibody (CBYC-P012) (CAT#: CBMAB-P0054-YC)

Human Recombinant AMBP protein, Fc Tag (CAT#: V2LY-0526-LY2355)

Human Recombinant AMBP protein, His Tag-1 (CAT#: V2LY-0526-LY2354)

Mouse Anti-AMBP Recombinant Antibody (1C11F7) (CAT#: CBMAB-R4261-CN)

AMBP Matched Antibody Pair (070) (CAT#: APMAB-070LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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