Mouse Anti-BCL2L10 Recombinant Antibody (CBT2381) (V2LY-0625-LY691)

Name: Mouse Anti-BCL2L10 Recombinant Antibody (CBT2381)
SKU: V2LY-0625-LY691
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT2381

Antibody Isotype

IgG2a

Application

Basic Information

Immunogen

Purified recombinant fragment of human BCL2L10 (AA: 31-186) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

BCL2-like 10 (apoptosis facilitator)

Entrez Gene ID

10017

UniProt ID

Q9HD36

Function

Promotes cell survival by suppressing apoptosis induced by BAX but not BAK (PubMed:11689480, PubMed:11278245).
Increases binding of AHCYL1/IRBIT to ITPR1 (PubMed:27995898).
Reduces ITPR1-mediated calcium release from the endoplasmic reticulum cooperatively with AHCYL1/IRBIT under normal cellular conditions (PubMed:27995898).
Under apoptotic stress conditions, dissociates from ITPR1 and is displaced from mitochondria-associated endoplasmic reticulum membranes, leading to increased Ca2+ transfer to mitochondria which promotes apoptosis (PubMed:27995898).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: ProtInc
Apoptotic process Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Female gamete generation Source: ProtInc
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Negative regulation of apoptotic process Source: HGNC-UCL
Negative regulation of intrinsic apoptotic signaling pathway Source: GO_Central
Spermatogenesis Source: ProtInc

Cellular Location

Nucleus membrane; Endoplasmic reticulum; Mitochondrion. Localizes to mitochondria-associated endoplasmic reticulum membranes (MAMs) (PubMed:27995898). Localization to MAMs is greatly reduced under apoptotic stress conditions (PubMed:27995898).

Topology

Helical: 183-200 aa

More Infomation

References

Lee, S. Y., Kwon, J., & Lee, K. A. (2021). Bcl2l10 induces metabolic alterations in ovarian cancer cells by regulating the TCA cycle enzymes SDHD and IDH1. Oncology reports, 45(4), 1-15.

Liu, F., Pan, R., Ding, H., Gu, L., Yang, Y., Li, C., ... & Nie, Y. (2021). UBQLN4 is an ATM substrate that stabilizes the anti‐apoptotic proteins BCL2A1 and BCL2L10 in mesothelioma. Molecular Oncology.

Quezada, M. J., Picco, M. E., Villanueva, M. B., Castro, M. V., Barbero, G., Fernández, N. B., ... & Lopez-Bergami, P. (2021). BCL2L10 Is Overexpressed in Melanoma Downstream of STAT3 and Promotes Cisplatin and ABT-737 Resistance. Cancers, 13(1), 78.

Lee, S. Y., Kwon, J., Woo, J. H., Kim, K. H., & Lee, K. A. (2020). Bcl2l10 mediates the proliferation, invasion and migration of ovarian cancer cells. International journal of oncology, 56(2), 618-629.

Huang, Y. L., Wang, H. J., Chen, F. M., Zhao, X. L., Fu, Q., Zhang, P. F., ... & Zhang, M. (2018). Role of BCL2L10 in regulating buffalo (Bubalus bubalis) oocyte maturation. Theriogenology, 110, 1-7.

Wang, X., Lu, J., Cao, J., Ma, B., Gao, C., & Qi, F. (2018). MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10. OncoTargets and therapy, 11, 7919.

Nougarede, A., Popgeorgiev, N., Kassem, L., Omarjee, S., Borel, S., Mikaelian, I., ... & Gillet, G. (2018). Breast cancer targeting through inhibition of the endoplasmic reticulum-based apoptosis regulator Nrh/BCL2L10. Cancer research, 78(6), 1404-1417.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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