Mouse Anti-BCL2L10 Recombinant Antibody (CBYY-0408) (CBMAB-0410-YY)

Name: Mouse Anti-BCL2L10 Recombinant Antibody (CBYY-0408)
SKU: CBMAB-0410-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0408

Application

ELISA, WB, FC

Immunogen

Purified recombinant fragment of human BCL2L10 (AA: 31-186) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:2,000
ELISA	1:100-1:2,000
FC	1:10-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BCL2-like 10 (apoptosis facilitator)

Introduction

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains conserved BH4, BH1 and BH2 domains. This protein can interact with other members of BCL-2 protein family including BCL2, BCL2L1/BCL-X(L), and BAX. Overexpression of this gene has been shown to suppress cell apoptosis possibly through the prevention of cytochrome C release from the mitochondria, and thus activating caspase-3 activation. The mouse counterpart of this protein is found to interact with Apaf1 and forMouse a protein complex with Caspase 9, which suggests the involvement of this protein in APAF1 and CASPASE 9 related apoptotic pathway.

Entrez Gene ID

10017

UniProt ID

Q9HD36

Alternative Names

BCL2 Like 10; BCL2-Like 10 (Apoptosis Facilitator); Anti-Apoptotic Protein NrH; Apoptosis Regulator Bcl-B; Bcl2-L-10; Death Inducer Binding To VBcl-2 And Apaf-1;

Function

Promotes cell survival by suppressing apoptosis induced by BAX but not BAK (PubMed:11689480, PubMed:11278245).
Increases binding of AHCYL1/IRBIT to ITPR1 (PubMed:27995898).
Reduces ITPR1-mediated calcium release from the endoplasmic reticulum cooperatively with AHCYL1/IRBIT under normal cellular conditions (PubMed:27995898).
Under apoptotic stress conditions, dissociates from ITPR1 and is displaced from mitochondria-associated endoplasmic reticulum membranes, leading to increased Ca2+ transfer to mitochondria which promotes apoptosis (PubMed:27995898).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: ProtInc
Apoptotic process Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Female gamete generation Source: ProtInc
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Negative regulation of apoptotic process Source: HGNC-UCL
Negative regulation of intrinsic apoptotic signaling pathway Source: GO_Central
Spermatogenesis Source: ProtInc

Cellular Location

Nucleus membrane; Endoplasmic reticulum; Mitochondrion. Localizes to mitochondria-associated endoplasmic reticulum membranes (MAMs) (PubMed:27995898). Localization to MAMs is greatly reduced under apoptotic stress conditions (PubMed:27995898).

Topology

Helical: 183-200 aa

Lee, S. Y., Kwon, J., & Lee, K. A. (2021). Bcl2l10 induces metabolic alterations in ovarian cancer cells by regulating the TCA cycle enzymes SDHD and IDH1. Oncology reports, 45(4), 1-15.

Liu, F., Pan, R., Ding, H., Gu, L., Yang, Y., Li, C., ... & Nie, Y. (2021). UBQLN4 is an ATM substrate that stabilizes the anti‐apoptotic proteins BCL2A1 and BCL2L10 in mesothelioma. Molecular Oncology.

Quezada, M. J., Picco, M. E., Villanueva, M. B., Castro, M. V., Barbero, G., Fernández, N. B., ... & Lopez-Bergami, P. (2021). BCL2L10 Is Overexpressed in Melanoma Downstream of STAT3 and Promotes Cisplatin and ABT-737 Resistance. Cancers, 13(1), 78.

Lee, S. Y., Kwon, J., Woo, J. H., Kim, K. H., & Lee, K. A. (2020). Bcl2l10 mediates the proliferation, invasion and migration of ovarian cancer cells. International journal of oncology, 56(2), 618-629.

Huang, Y. L., Wang, H. J., Chen, F. M., Zhao, X. L., Fu, Q., Zhang, P. F., ... & Zhang, M. (2018). Role of BCL2L10 in regulating buffalo (Bubalus bubalis) oocyte maturation. Theriogenology, 110, 1-7.

Wang, X., Lu, J., Cao, J., Ma, B., Gao, C., & Qi, F. (2018). MicroRNA-18a promotes hepatocellular carcinoma proliferation, migration, and invasion by targeting Bcl2L10. OncoTargets and therapy, 11, 7919.

Nougarede, A., Popgeorgiev, N., Kassem, L., Omarjee, S., Borel, S., Mikaelian, I., ... & Gillet, G. (2018). Breast cancer targeting through inhibition of the endoplasmic reticulum-based apoptosis regulator Nrh/BCL2L10. Cancer research, 78(6), 1404-1417.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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