Mouse Anti-CIAO1 Recombinant Antibody (0230) (CBMAB-0230-YC)

Name: Mouse Anti-CIAO1 Recombinant Antibody (0230)
SKU: CBMAB-0230-YC
Rating: 5 (1 reviews)

See all CIAO1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

0230

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-339 of Ciao 1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CIAO1

Introduction

Iron–sulfur (Fe–S) clusters are essential for numerous biological processes, including mitochondrial respiratory chain activity and various other enzymatic and regulatory functions. Human Fe–S cluster assembly proteins are frequently encoded by single genes, and inherited defects in some of these genes cause disease. CIAO1 (cytosolic iron-sulfur assembly component 1) is a protein that in humans is encoded by the CIAO1 gene.

Entrez Gene ID

9391

UniProt ID

O76071

Alternative Names

CIA1; WDR39

Function

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:17937914, PubMed:23891004).

As a CIA complex component, interacts specifically with CIAO2A or CIAO2B and MMS19 to assist different branches of iron-sulfur protein assembly, depending of its interactors. The complex CIAO1:CIAO2B:MMS19 binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. CIAO1:CIAO2A specifically matures ACO1 and stabilizes IREB2 (PubMed:23891004).

Seems to specifically modulate the transactivation activity of WT1 (PubMed:9556563).

As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed:20797633).

Biological Process

Chromosome segregation Source: UniProtKB-KW
Iron-sulfur cluster assembly Source: UniProtKB
Positive regulation of cell population proliferation Source: ProtInc
Protein maturation by iron-sulfur cluster transfer Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: ProtInc

Cellular Location

Cytoplasm

Kim, K. S., Maio, N., Singh, A., & Rouault, T. A. (2018). Cytosolic HSC20 integrates de novo iron–sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Human molecular genetics, 27(5), 837-852.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CIAO1 Recombinant Antibody (0230)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-CIAO1 Recombinant Antibody (6C9) (CAT#: CBMAB-C0001-WJ)

Mouse Anti-CIAO1 Recombinant Antibody (CBXC-2998) (CAT#: CBMAB-C0314-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry