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Mouse Anti-CYP2C19 Recombinant Antibody (EG895) (V2LY-0125-LY953)


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Summary

Host Animal
Mouse
Specificity
Human, Rat, Monkey, Mouse
Clone
EG895
Antibody Isotype
IgG1
Application
WB, IHC, FC

Basic Information

Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cytochrome p450 2C19/CYP2C19.
Host Species
Mouse
Specificity
Human, Rat, Monkey, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
FC1-3 μg/10⁶ cells
WB0.1-0.5 μg/mL
IHC0.5-1 μg/mL

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS & Trehalose
Preservative
Sodium Azide
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 2 Subfamily C Member 19
Entrez Gene ID
1557
UniProt ID
P33261
Function
A cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) (PubMed:18577768, PubMed:19965576, PubMed:20972997).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:18577768, PubMed:19965576, PubMed:20972997).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates PUFA specifically at the omega-1 position (PubMed:18577768).

Catalyzes the epoxidation of double bonds of PUFA (PubMed:20972997, PubMed:19965576).

Also metabolizes plant monoterpenes such as limonene. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol (PubMed:11950794).

Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine. Hydroxylates fenbendazole at the 4' position (PubMed:23959307).
Biological Process
Drug metabolic process Source: BHF-UCL
Epoxygenase P450 pathway Source: GO_Central
Exogenous drug catabolic process Source: BHF-UCL
Heterocycle metabolic process Source: BHF-UCL
Monoterpenoid metabolic process Source: BHF-UCL
Omega-hydroxylase P450 pathway Source: Reactome
Organic acid metabolic process Source: GO_Central
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
More Infomation

Botton, M. R., Whirl‐Carrillo, M., Del Tredici, A. L., Sangkuhl, K., Cavallari, L. H., Agúndez, J. A., ... & Gaedigk, A. (2021). PharmVar GeneFocus: CYP2C19. Clinical Pharmacology & Therapeutics, 109(2), 352-366.

Koopmans, A. B., Braakman, M. H., Vinkers, D. J., Hoek, H. W., & van Harten, P. N. (2021). Meta-analysis of probability estimates of worldwide variation of CYP2D6 and CYP2C19. Translational psychiatry, 11(1), 1-16.

Wang, Y., Meng, X., Wang, A., Xie, X., Pan, Y., Johnston, S. C., ... & Wang, B. (2021). Ticagrelor versus clopidogrel in CYP2C19 loss-of-function carriers with stroke or TIA. New England Journal of Medicine, 385(27), 2520-2530.

Pratt, V. M., Del Tredici, A. L., Hachad, H., Ji, Y., Kalman, L. V., Scott, S. A., & Weck, K. E. (2018). Recommendations for clinical CYP2C19 genotyping allele selection: a report of the association for molecular pathology. The Journal of Molecular Diagnostics, 20(3), 269-276.

Cavallari, L. H., Lee, C. R., Beitelshees, A. L., Cooper-DeHoff, R. M., Duarte, J. D., Voora, D., ... & IGNITE Network. (2018). Multisite investigation of outcomes with implementation of CYP2C19 genotype-guided antiplatelet therapy after percutaneous coronary intervention. JACC: Cardiovascular Interventions, 11(2), 181-191.

Dean, L. (2018). Clopidogrel therapy and CYP2C19 genotype. Medical Genetics Summaries [Internet].

El Rouby, N., Lima, J. J., & Johnson, J. A. (2018). Proton pump inhibitors: from CYP2C19 pharmacogenetics to precision medicine. Expert opinion on drug metabolism & toxicology, 14(4), 447-460.

Jukić, M. M., Haslemo, T., Molden, E., & Ingelman-Sundberg, M. (2018). Impact of CYP2C19 genotype on escitalopram exposure and therapeutic failure: a retrospective study based on 2,087 patients. American Journal of Psychiatry, 175(5), 463-470.

Empey, P. E., Stevenson, J. M., Tuteja, S., Weitzel, K. W., Angiolillo, D. J., Beitelshees, A. L., ... & IGNITE Network. (2018). Multisite investigation of strategies for the implementation of CYP2C19 genotype‐guided antiplatelet therapy. Clinical Pharmacology & Therapeutics, 104(4), 664-674.

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For research use only. Not intended for any clinical use.

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