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Rabbit Anti-CYP2D6 Recombinant Antibody (C3309) (V2LY-0125-LY960)


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Summary

Host Animal
Rabbit
Specificity
Human
Clone
C3309
Antibody Isotype
IgG
Application
WB, IHC, FC, IF

Basic Information

Immunogen
Recombinant protein within Human Cytochrome P450 2D6 aa 301-497.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IHC-P1:50-1:200
WB1:500-1:2,000
IF(ICC)1:50-1:200
FC1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA, Glycerol & TBS
Preservative
Sodium Azide
Concentration
1 mg/mL
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome P450 Family 2 Subfamily D Member 6
Entrez Gene ID
1565
UniProt ID
P10635
Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids, steroids and retinoids (PubMed:18698000, PubMed:19965576, PubMed:20972997, PubMed:21289075, PubMed:21576599).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:18698000, PubMed:19965576, PubMed:20972997, PubMed:21289075, PubMed:21576599).

Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) (PubMed:19965576, PubMed:20972997).

Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 20-hydroxyeicosatetraenoic acid ethanolamide (20-HETE-EA) and 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed:18698000, PubMed:21289075).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599).

Catalyzes the oxidative transformations of all-trans retinol to all-trans retinal, a precursor for the active form all-trans-retinoic acid (PubMed:10681376).

Also involved in the oxidative metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants.
Biological Process
Alkaloid catabolic process Source: BHF-UCL
Alkaloid metabolic process Source: BHF-UCL
Arachidonic acid metabolic process Source: GO_Central
Cholesterol metabolic process Source: UniProtKB-UniPathway
Coumarin metabolic process Source: BHF-UCL
Drug catabolic process Source: BHF-UCL
Drug metabolic process Source: BHF-UCL
Estrogen metabolic process Source: UniProtKB
Exogenous drug catabolic process Source: GO_Central
Heterocycle metabolic process Source: BHF-UCL
Isoquinoline alkaloid metabolic process Source: BHF-UCL
Long-chain fatty acid biosynthetic process Source: Reactome
Monoterpenoid metabolic process Source: BHF-UCL
Negative regulation of binding Source: BHF-UCL
Negative regulation of cellular organofluorine metabolic process Source: BHF-UCL
Organic acid metabolic process Source: GO_Central
Oxidative demethylation Source: BHF-UCL
Retinol metabolic process Source: UniProtKB
Steroid metabolic process Source: BHF-UCL
Xenobiotic metabolic process Source: GO_Central
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
More Infomation

van der Lee, M., Allard, W. G., Vossen, R. H., Baak-Pablo, R. F., Menafra, R., Deiman, B. A., ... & Anvar, S. Y. (2021). Toward predicting CYP2D6-mediated variable drug response from CYP2D6 gene sequencing data. Science translational medicine, 13(603), eabf3637.

Koopmans, A. B., Braakman, M. H., Vinkers, D. J., Hoek, H. W., & van Harten, P. N. (2021). Meta-analysis of probability estimates of worldwide variation of CYP2D6 and CYP2C19. Translational psychiatry, 11(1), 1-16.

Nofziger, C., Turner, A. J., Sangkuhl, K., Whirl‐Carrillo, M., Agúndez, J. A., Black, J. L., ... & Gaedigk, A. (2020). PharmVar GeneFocus: CYP2D6. Clinical Pharmacology & Therapeutics, 107(1), 154-170.

Taylor, C., Crosby, I., Yip, V., Maguire, P., Pirmohamed, M., & Turner, R. M. (2020). A Review of the Important Role of CYP2D6 in Pharmacogenomics. Genes, 11(11), 1295.

Smith, D. M., Weitzel, K. W., Elsey, A. R., Langaee, T., Gong, Y., Wake, D. T., ... & Cavallari, L. H. (2019). CYP2D6-guided opioid therapy improves pain control in CYP2D6 intermediate and poor metabolizers: a pragmatic clinical trial. Genetics in Medicine, 21(8), 1842-1850.

Del Tredici, A. L., Malhotra, A., Dedek, M., Espin, F., Roach, D., Zhu, G. D., ... & Moreno, T. A. (2018). Frequency of CYP2D6 alleles including structural variants in the United States. Frontiers in pharmacology, 9, 305.

Gaedigk, A., Dinh, J. C., Jeong, H., Prasad, B., & Leeder, J. S. (2018). Ten years’ experience with the CYP2D6 activity score: a perspective on future investigations to improve clinical predictions for precision therapeutics. Journal of personalized medicine, 8(2), 15.

Pan, X., Ning, M., & Jeong, H. (2017). Transcriptional regulation of CYP2D6 expression. Drug Metabolism and Disposition, 45(1), 42-48.

Gaedigk, A., Sangkuhl, K., Whirl-Carrillo, M., Klein, T., & Leeder, J. S. (2017). Prediction of CYP2D6 phenotype from genotype across world populations. Genetics in Medicine, 19(1), 69-76.

Dean, L. (2017). Codeine therapy and CYP2D6 genotype.

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For research use only. Not intended for any clinical use.

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