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Mouse Anti-DIXDC1 Recombinant Antibody (1B3) (V2LY-0425-LY378)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
1B3
Antibody Isotype
IgG2a
Application
IF, WB

Basic Information

Immunogen
Full length human recombinant protein of human DIXDC1 produced in HEK293T cell.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:2,000
IF(ICC)1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & PBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
85458
UniProt ID
Q155Q3
Function
Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
Biological Process
Canonical Wnt signaling pathway Source: UniProtKB
Cerebral cortex radially oriented cell migration Source: Ensembl
Forebrain ventricular zone progenitor cell division Source: Ensembl
Negative regulation of neuron differentiation Source: Ensembl
Positive regulation of Wnt signaling pathway Source: Ensembl
Regulation of actin cytoskeleton organization Source: Ensembl
Regulation of microtubule cytoskeleton organization Source: Ensembl
Cellular Location
Cytoplasm; Stress fiber; Focal adhesion. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis (PubMed:19375513). Associated with actin stress fiber at the filament ends (PubMed:16814745).
Isoform 2: Cytoplasm. Not associated with stress fibers.
PTM
Phosphorylated on tyrosine and serine residues.
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.
More Infomation

Kim, Y., Kim, D. Y., Zhang, H., Bae, C. R., Seong, D., Kim, Y., ... & Kwon, Y. G. (2022). DIX domain containing 1 (DIXDC1) modulates VEGFR2 level in vasculatures to regulate embryonic and postnatal retina angiogenesis. BMC biology, 20(1), 1-18.

Kwan, V., Rosa, E., Xing, S., Murtaza, N., Singh, K., Holzapfel, N. T., ... & Singh, K. K. (2020). Proteomic Analysis Reveals Autism-Associated Gene DIXDC1 Regulates Proteins Associated with Mitochondrial Organization and Function. Journal of Proteome Research, 20(1), 1052-1062.

Chen, Z. F., Wang, Y., Sun, L. L., Ding, S. Y., & Jinag, H. (2020). LncRNA SNHG20 enhances the progression of oral squamous cell carcinoma by regulating the miR-29a/DIXDC1/Wnt regulatory axis. Eur Rev Med Pharmacol Sci, 24(10), 5436-45.

Song, J., Guan, Z., Li, M., Sha, S., Song, C., Gao, Z., & Zhao, Y. (2018). MicroRNA-154 inhibits the growth and invasion of gastric cancer cells by targeting DIXDC1/WNT signaling. Oncology research, 26(6), 847.

Xin, H., Li, C., & Wang, M. (2018). DIXDC1 promotes the growth of acute myeloid leukemia cells by upregulating the Wnt/β-catenin signaling pathway. Biomedicine & Pharmacotherapy, 107, 1548-1555.

Che, X., Qian, Y., & Li, D. (2018). Suppression of Disheveled–Axin Domain Containing 1 (DIXDC1) by microRNA-186 inhibits the proliferation and invasion of retinoblastoma cells. Journal of Molecular Neuroscience, 64(2), 252-261.

Martin, P. M., Stanley, R. E., Ross, A. P., Freitas, A. E., Moyer, C. E., Brumback, A. C., ... & Cheyette, B. N. (2018). DIXDC1 contributes to psychiatric susceptibility by regulating dendritic spine and glutamatergic synapse density via GSK3 and Wnt/β-catenin signaling. Molecular psychiatry, 23(2), 467-475.

Zhong, J., Liu, Y., Xu, Q., Yu, J., & Zhang, M. (2017). Inhibition of DIXDC1 by microRNA-1271 suppresses the proliferation and invasion of prostate cancer cells. Biochemical and biophysical research communications, 484(4), 794-800.

Chen, J., Shen, C., Shi, J., Shen, J., Chen, W., Sun, J., ... & Huang, Q. (2017). Knockdown of DIXDC1 inhibits the proliferation and migration of human glioma cells. Cellular and molecular neurobiology, 37(6), 1009-1019.

Lu, H., Jiang, R., Tao, X., Duan, C., Huang, J., Huan, W., ... & Ren, J. (2017). Expression of Dixdc1 and its role in astrocyte proliferation after traumatic brain injury. Cellular and Molecular Neurobiology, 37(6), 1131-1139.

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For research use only. Not intended for any clinical use.

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