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Mouse Anti-DIXDC1 Recombinant Antibody (V2-32858) (CBMAB-D0958-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DIX Domain Containing 1 (DIXDC1). The antibody can be used for immunoassay techniques, such as WB.
See all DIXDC1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-32858
Antibody Isotype
IgG1
Application
WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DIX Domain Containing 1
Introduction
DIXDC1 is a positive regulator of the Wnt signaling pathway. The protein is found associated with gamma tubulin at the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Entrez Gene ID
85458
UniProt ID
Q155Q3
Alternative Names
DIX Domain Containing 1; Coiled-Coil Protein DIX1; CCD1; DIX Domain-Containing Protein 1; Coiled-Coil-DIX1; KIAA1735; Dixin;
Function
Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
Biological Process
Canonical Wnt signaling pathway Source: UniProtKB
Cerebral cortex radially oriented cell migration Source: Ensembl
Forebrain ventricular zone progenitor cell division Source: Ensembl
Negative regulation of neuron differentiation Source: Ensembl
Positive regulation of Wnt signaling pathway Source: Ensembl
Regulation of actin cytoskeleton organization Source: Ensembl
Regulation of microtubule cytoskeleton organization Source: Ensembl
Cellular Location
Cytoplasm; Stress fiber; Focal adhesion. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis (PubMed:19375513). Associated with actin stress fiber at the filament ends (PubMed:16814745).
Isoform 2: Cytoplasm. Not associated with stress fibers.
PTM
Phosphorylated on tyrosine and serine residues.
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.

Kim, Y., Kim, D. Y., Zhang, H., Bae, C. R., Seong, D., Kim, Y., ... & Kwon, Y. G. (2022). DIX domain containing 1 (DIXDC1) modulates VEGFR2 level in vasculatures to regulate embryonic and postnatal retina angiogenesis. BMC biology, 20(1), 1-18.

Kwan, V., Rosa, E., Xing, S., Murtaza, N., Singh, K., Holzapfel, N. T., ... & Singh, K. K. (2020). Proteomic Analysis Reveals Autism-Associated Gene DIXDC1 Regulates Proteins Associated with Mitochondrial Organization and Function. Journal of Proteome Research, 20(1), 1052-1062.

Chen, Z. F., Wang, Y., Sun, L. L., Ding, S. Y., & Jinag, H. (2020). LncRNA SNHG20 enhances the progression of oral squamous cell carcinoma by regulating the miR-29a/DIXDC1/Wnt regulatory axis. Eur Rev Med Pharmacol Sci, 24(10), 5436-45.

Song, J., Guan, Z., Li, M., Sha, S., Song, C., Gao, Z., & Zhao, Y. (2018). MicroRNA-154 inhibits the growth and invasion of gastric cancer cells by targeting DIXDC1/WNT signaling. Oncology research, 26(6), 847.

Xin, H., Li, C., & Wang, M. (2018). DIXDC1 promotes the growth of acute myeloid leukemia cells by upregulating the Wnt/β-catenin signaling pathway. Biomedicine & Pharmacotherapy, 107, 1548-1555.

Che, X., Qian, Y., & Li, D. (2018). Suppression of Disheveled–Axin Domain Containing 1 (DIXDC1) by microRNA-186 inhibits the proliferation and invasion of retinoblastoma cells. Journal of Molecular Neuroscience, 64(2), 252-261.

Martin, P. M., Stanley, R. E., Ross, A. P., Freitas, A. E., Moyer, C. E., Brumback, A. C., ... & Cheyette, B. N. (2018). DIXDC1 contributes to psychiatric susceptibility by regulating dendritic spine and glutamatergic synapse density via GSK3 and Wnt/β-catenin signaling. Molecular psychiatry, 23(2), 467-475.

Zhong, J., Liu, Y., Xu, Q., Yu, J., & Zhang, M. (2017). Inhibition of DIXDC1 by microRNA-1271 suppresses the proliferation and invasion of prostate cancer cells. Biochemical and biophysical research communications, 484(4), 794-800.

Chen, J., Shen, C., Shi, J., Shen, J., Chen, W., Sun, J., ... & Huang, Q. (2017). Knockdown of DIXDC1 inhibits the proliferation and migration of human glioma cells. Cellular and molecular neurobiology, 37(6), 1009-1019.

Lu, H., Jiang, R., Tao, X., Duan, C., Huang, J., Huan, W., ... & Ren, J. (2017). Expression of Dixdc1 and its role in astrocyte proliferation after traumatic brain injury. Cellular and Molecular Neurobiology, 37(6), 1131-1139.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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