Mouse Anti-EBF3 Recombinant Antibody (CBFYE-0013) (V2LY-0425-LY1279)

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Basic Information

Host Animal
Mouse
Clone
CBFYE-0013
Application
WB, ELISA
Immunogen
EBF3 (aa381-480) partial recombinant protein.
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
early B-cell factor 3
Entrez Gene ID
Human253738
Rat361668
UniProt ID
HumanQ9H4W6
RatD4A274
Research Area
Transcriptional activator (PubMed:28017373, PubMed:28017372, PubMed:28017370).

Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (By similarity).
Biological Process
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
Hypotonia, ataxia, and delayed development syndrome (HADDS):
An autosomal dominant neurodevelopmental syndrome characterized by global developmental delay, moderate to severe intellectual disability, cerebellar ataxia, hypotonia, speech delay, variable dysmorphic features, and genitourinary abnormalities including vesicoureteric reflux.

Deisseroth, C. A., Lerma, V. C., Magyar, C. L., Pfliger, J. M., Nayak, A., Bliss, N. D., ... & Chao, H. T. (2022). An Integrated Phenotypic and Genotypic Approach Reveals a High‐Risk Subtype Association for EBF3 Missense Variants Affecting the Zinc Finger Domain. Annals of Neurology.

Ding, S., Wang, X., Lv, D., Tao, Y., Liu, S., Chen, C., ... & Xia, Y. (2022). EBF3 reactivation by inhibiting the EGR1/EZH2/HDAC9 complex promotes metastasis via transcriptionally enhancing vimentin in nasopharyngeal carcinoma. Cancer Letters, 527, 49-65.

Ignatius, E., Puosi, R., Palomäki, M., Forsbom, N., Pohjanpelto, M., Alitalo, T., ... & Isohanni, P. (2022). Duplication/triplication mosaicism of EBF3 and expansion of the EBF3 neurodevelopmental disorder phenotype. European Journal of Paediatric Neurology, 37, 1-7.

de la Peña, M. J., de Domingo, A. J., Tirado, P., Calleja-Pérez, B., Alcaraz, L. A., Álvarez, S., ... & Fernández-Jaén, A. (2021). Neuroimaging findings in patients with EBF3 mutations: report of two cases. Molecular Syndromology, 12(3), 186-193.

Kuriki, M., Sato, F., Arai, H. N., Sogabe, M., Kaneko, M., Kiyonari, H., ... & Sehara-Fujisawa, A. (2020). Transient and lineage-restricted requirement of Ebf3 for sternum ossification. Development, 147(9), d

Iwai, R., Tabata, H., Inoue, M., Nomura, K. I., Okamoto, T., Ichihashi, M., ... & Mizutani, K. I. (2018). A Prdm8 target gene Ebf3 regulates multipolar-to-bipolar transition in migrating neocortical cells. Biochemical and biophysical research communications, 495(1), 388-394.

Seike, M., Omatsu, Y., Watanabe, H., Kondoh, G., & Nagasawa, T. (2018). Stem cell niche-specific Ebf3 maintains the bone marrow cavity. Genes & Development, 32(5-6), 359-372.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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