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Mouse Anti-EIF5 Recombinant Antibody (CBT4715) (V2LY-0625-LY552)


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Summary

Host Animal
Mouse
Specificity
Human, Monkey
Clone
CBT4715
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Monkey
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 5
Entrez Gene ID
1983
UniProt ID
P55010
Research Area
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Biological Process
Activation of GTPase activity Source: Ensembl
Formation of cytoplasmic translation initiation complex Source: GO_Central
Formation of translation preinitiation complex Source: GO_Central
Regulation of translational initiation Source: CACAO
Cellular Location
Cytosol; Plasma membrane; Cytoplasm
More Infomation

Li, Z., Wu, Y., Fu, Y., Chen, X., Zhao, X., Wu, X., ... & Sun, F. (2022). Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex. Stem cell research & therapy, 13(1), 1-18.

Paul, E. E., Lin, K. Y., Gamble, N., Tsai, A. W. L., Swan, S. H., Yang, Y., ... & Marintchev, A. (2022). Dynamic interaction network involving the conserved intrinsically disordered regions in human eIF5. Biophysical Chemistry, 281, 106740.

Singh, C. R., Jaiswal, R., Escalante, C. R., & Asano, K. (2022). Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein. STAR protocols, 3(3), 101615.

Marintchev, A., Paul, E. E., Lin, K. Y., Tsai, A. W. L., Yang, Y., Gamble, N., & Doran, M. (2022). Dynamic Interaction Network Involving the Conserved Intrinsically Disordered Regions in Human eIF5. The FASEB Journal, 36.

Cai, S. X., Chen, W. S., Zeng, W., Cheng, X. F., Lin, M. B., & Wang, J. S. (2021). Roles of HDAC2, eIF5, and eIF6 in lung cancer tumorigenesis. Current medical science, 41(4), 764-769.

Zeman, J., Itoh, Y., Kukačka, Z., Rosůlek, M., Kavan, D., Kouba, T., ... & Valášek, L. S. (2019). Binding of eIF3 in complex with eIF5 and eIF1 to the 40S ribosomal subunit is accompanied by dramatic structural changes. Nucleic acids research, 47(15), 8282-8300.

Lin, K. Y., Nag, N., Pestova, T. V., & Marintchev, A. (2018). Human eIF5 and eIF1A compete for binding to eIF5B. Biochemistry, 57(40), 5910-5920.

Llácer, J. L., Hussain, T., Saini, A. K., Nanda, J. S., Kaur, S., Gordiyenko, Y., ... & Ramakrishnan, V. (2018). Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition. Elife, 7, e39273.

Ross, J. A., Bressler, K. R., & Thakor, N. (2018). Eukaryotic initiation factor 5B (eIF5B) cooperates with eIF1A and eIF5 to facilitate uORF2-mediated repression of ATF4 translation. International journal of molecular sciences, 19(12), 4032.

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For research use only. Not intended for any clinical use.

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