Mouse Anti-EPN1 Recombinant Antibody (E0232) (V2LY-0825-LY528)

Name: Mouse Anti-EPN1 Recombinant Antibody (E0232)
SKU: V2LY-0825-LY528
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

E0232

Application

ELISA, WB, IP, IF

Immunogen

Amino acids 550-575 at the C-terminus of rat epsin 1.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Cattle

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Epsin 1

Entrez Gene ID

Human	29924
Mouse	13854

UniProt ID

Human	Q9Y6I3
Mouse	Q80VP1

Research Area

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity).

Regulates receptor-mediated endocytosis (PubMed:10557078, PubMed:10393179).

Biological Process

Embryonic organ development Source: Ensembl
Endocytosis Source: GO_Central
Female pregnancy Source: Ensembl
In utero embryonic development Source: Ensembl
Negative regulation of sprouting angiogenesis Source: BHF-UCL
Notch signaling pathway Source: Ensembl

Cellular Location

Cell membrane; Nucleus; Cytoplasm; Clathrin-coated pit. Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity).

PTM

Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
Ubiquitinated.

Song, K., Cai, X., Dong, Y., Wu, H., Wei, Y., Shankavaram, U. T., ... & Chen, H. (2021). Epsins 1 and 2 promote NEMO linear ubiquitination via LUBAC to drive breast cancer development. The Journal of clinical investigation, 131(1).

Kroppen, B., Teske, N., Yambire, K. F., Denkert, N., Mukherjee, I., Tarasenko, D., ... & Meinecke, M. (2021). Cooperativity of membrane-protein and protein–protein interactions control membrane remodeling by epsin 1 and affects clathrin-mediated endocytosis. Cellular and Molecular Life Sciences, 78(5), 2355-2370.

Brophy, M. L., Dong, Y., Tao, H., Yancey, P. G., Song, K., Zhang, K., ... & Chen, H. (2019). Myeloid-specific deletion of epsins 1 and 2 reduces atherosclerosis by preventing LRP-1 downregulation. Circulation research, 124(4), e6-e19.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-EPN1 Recombinant Antibody (CBT3298) (CAT#: V2LY-0625-LY173)

Mouse Anti-EPN1 Recombinant Antibody (XB0401) (CAT#: V2LY-0825-LY529)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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