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Mouse Anti-EPOR Recombinant Antibody (3D10) (CBMAB-A2673-LY)

The product is antibody recognizes EPOR. The antibody 3D10 immunoassay techniques such as: WB, ELISA.
See all EPOR antibodies
Published Data
Fig.1 The expression of erythropoietin receptor (EPOR) in C6 cells. Immunofluorescence staining of C6 cells with an anti-EPOR antibody was performed. The EPOR was well expressed in the C6 cells derived from rat astrocytes.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D10
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
EPOR (NP_000112, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Erythropoietin Receptor
Introduction
The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. [provided by RefSeq]
Entrez Gene ID
2057
UniProt ID
P19235
Alternative Names
MGC138358
Research Area
Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.
Biological Process
Brain development Source: Ensembl
Decidualization Source: Ensembl
Heart development Source: Ensembl
Signal transduction Source: ProtInc
Cellular Location
Cell membrane
Isoform EPOR-S: Secreted. Secreted and located to the cell surface.
Involvement in disease
Erythrocytosis, familial, 1 (ECYT1):
An autosomal dominant disorder characterized by elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.
Topology
Extracellular: 25-250
Helical: 251-273
Cytoplasmic: 274-508
PTM
On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation (By similarity). Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

Mochida, Y., Hara, A., Oka, M., Maesato, K., Ishioka, K., Moriya, H., ... & Wada, T. (2022). Association between Anti-Erythropoietin Receptor Antibodies and Cardiac Function in Patients on Hemodialysis: A Multicenter Cross-Sectional Study. Biomedicines, 10(9), 2092.

Papic, M., Zivanovic, S., Vucicevic, T., Papic, M. V., Zdravkovic, D., Milivojevic, N., ... & Popovic, M. (2022). Pulpal expression of erythropoietin and erythropoietin receptor after direct pulp capping in rat. European Journal of Oral Sciences, e12888.

Ramshekar, A., Bretz, C. A., Kunz, E., Cung, T., Richards, B. T., Stoddard, G. J., ... & Hartnett, M. E. (2022). Role of Erythropoietin Receptor Signaling in Macrophages or Choroidal Endothelial Cells in Choroidal Neovascularization. Biomedicines, 10(7), 1655.

Nijholt, K. T., Meems, L. M., Ruifrok, W., Maass, A. H., Yurista, S. R., Pavez-Giani, M. G., ... & Westenbrink, B. D. (2021). The erythropoietin receptor expressed in skeletal muscle is essential for mitochondrial biogenesis and physiological exercise. Pflügers Archiv-European Journal of Physiology, 473(8), 1301-1313.

Oshima, M., Hara, A., Toyama, T., Jun, M., Pollock, C., Jardine, M., ... & Wada, T. (2021). Comparison of circulating biomarkers in predicting diabetic kidney disease progression with autoantibodies to erythropoietin receptor. Kidney international reports, 6(2), 284-295.

Vukelic, J., Dobrila-Dintinjana, R., Marijic, B., Marzic, D., Braut, T., & Velepic, M. (2021). New insights into erythropoietin and erythropoietin receptor in laryngeal cancer tissue. Medicine, 100(3).

Wu, B. W., Liu, Y., Wu, M. S., Meng, Y. H., Lu, M., Guo, J. D., & Zhou, Y. H. (2020). Downregulation of microRNA‐135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor. IUBMB life, 72(2), 198-213.

Bretz, C. A., Simmons, A. B., Kunz, E., Ramshekar, A., Kennedy, C., Cardenas, I., & Hartnett, M. E. (2020). Erythropoietin receptor signaling supports retinal function after vascular injury. The American journal of pathology, 190(3), 630-641.

Shi, M., Flores, B., Li, P., Gillings, N., McMillan, K. L., Ye, J., ... & Hu, M. C. (2018). Effects of erythropoietin receptor activity on angiogenesis, tubular injury, and fibrosis in acute kidney injury: a “U-shaped” relationship. American Journal of Physiology-Renal Physiology, 314(4), F501-F516.

Ostrowski, D., & Heinrich, R. (2018). Alternative erythropoietin receptors in the nervous system. Journal of Clinical Medicine, 7(2), 24.

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For research use only. Not intended for any clinical use.

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