Rabbit Anti-ERAP1 Recombinant Antibody (E1622) (V2LY-0825-LY582)

Name: Rabbit Anti-ERAP1 Recombinant Antibody (E1622)
SKU: V2LY-0825-LY582
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

E1622

Application

WB, IHC-P

Immunogen

Recombinant protein within human erap1 aa 833-941.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IHC-P	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

TBS, glycerol, BSA

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Endoplasmic Reticulum Aminopeptidase 1

Entrez Gene ID

Human	51752
Mouse	80898
Rat	80897

UniProt ID

Human	Q9NZ08
Mouse	Q9EQH2
Rat	Q9JJ22

Research Area

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Angiogenesis Source: HGNC-UCL
Antigen processing and presentation of endogenous peptide antigen via MHC class I Source: HGNC-UCL
Antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
Fat cell differentiation Source: UniProtKB
Membrane protein ectodomain proteolysis Source: UniProtKB
Peptide catabolic process Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Proteolysis Source: GO_Central
Regulation of blood pressure Source: GO_Central
Regulation of innate immune response Source: UniProtKB
Response to bacterium Source: BHF-UCL
Signal transduction Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1
Helical: 2-21
Lumenal: 22-941

PTM

N-glycosylated.

Arakawa, A., Reeves, E., Vollmer, S., Arakawa, Y., He, M., Galinski, A., ... & Prinz, J. C. (2021). ERAP1 controls the autoimmune response against melanocytes in psoriasis by generating the melanocyte autoantigen and regulating its amount for HLA-C* 06: 02 presentation. The Journal of Immunology, 207(9), 2235-2244.

Maben, Z., Arya, R., Georgiadis, D., Stratikos, E., & Stern, L. J. (2021). Conformational dynamics linked to domain closure and substrate binding explain the ERAP1 allosteric regulation mechanism. Nature communications, 12(1), 1-15.

Reeves, E., Islam, Y., & James, E. (2020). ERAP1: A potential therapeutic target for a myriad of diseases. Expert Opinion on Therapeutic Targets, 24(6), 535-544.

Babaie, F., Hosseinzadeh, R., Ebrazeh, M., Seyfizadeh, N., Aslani, S., Salimi, S., ... & Mohammadi, H. (2020). The roles of ERAP1 and ERAP2 in autoimmunity and cancer immunity: New insights and perspective. Molecular immunology, 121, 7-19.

Bufalieri, F., Infante, P., Bernardi, F., Caimano, M., Romania, P., Moretti, M., ... & Di Marcotullio, L. (2019). ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Nature communications, 10(1), 1-15.

Compagnone, M., Cifaldi, L., & Fruci, D. (2019). Regulation of ERAP1 and ERAP2 genes and their disfunction in human cancer. Human immunology, 80(5), 318-324.

Admon, A. (2019). ERAP1 shapes just part of the immunopeptidome. Human immunology, 80(5), 296-301.

Yao, Y., Liu, N., Zhou, Z., & Shi, L. (2019). Influence of ERAP1 and ERAP2 gene polymorphisms on disease susceptibility in different populations. Human immunology, 80(5), 325-334.

Reeves, E., & James, E. (2018). The role of polymorphic ERAP1 in autoinflammatory disease. Bioscience reports, 38(4).

López de Castro, J. A. (2018). How ERAP1 and ERAP2 shape the peptidomes of disease-associated MHC-I proteins. Frontiers in Immunology, 9, 2463.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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