Rat Anti-ERAP1 Recombinant Antibody (EG1121) (V2LY-0825-LY585)

Name: Rat Anti-ERAP1 Recombinant Antibody (EG1121)
SKU: V2LY-0825-LY585
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

EG1121

Application

Immunogen

Recombinant mouse ERAP1 (Ser27-Leu930).

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Endoplasmic Reticulum Aminopeptidase 1

Entrez Gene ID

80898

UniProt ID

Q9EQH2

Research Area

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Angiogenesis Source: HGNC-UCL
Antigen processing and presentation of endogenous peptide antigen via MHC class I Source: HGNC-UCL
Antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
Fat cell differentiation Source: UniProtKB
Membrane protein ectodomain proteolysis Source: UniProtKB
Peptide catabolic process Source: GO_Central
Positive regulation of angiogenesis Source: Ensembl
Proteolysis Source: GO_Central
Regulation of blood pressure Source: GO_Central
Regulation of innate immune response Source: UniProtKB
Response to bacterium Source: BHF-UCL
Signal transduction Source: GO_Central

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1
Helical: 2-21
Lumenal: 22-941

PTM

N-glycosylated.

Arakawa, A., Reeves, E., Vollmer, S., Arakawa, Y., He, M., Galinski, A., ... & Prinz, J. C. (2021). ERAP1 controls the autoimmune response against melanocytes in psoriasis by generating the melanocyte autoantigen and regulating its amount for HLA-C* 06: 02 presentation. The Journal of Immunology, 207(9), 2235-2244.

Maben, Z., Arya, R., Georgiadis, D., Stratikos, E., & Stern, L. J. (2021). Conformational dynamics linked to domain closure and substrate binding explain the ERAP1 allosteric regulation mechanism. Nature communications, 12(1), 1-15.

Reeves, E., Islam, Y., & James, E. (2020). ERAP1: A potential therapeutic target for a myriad of diseases. Expert Opinion on Therapeutic Targets, 24(6), 535-544.

Babaie, F., Hosseinzadeh, R., Ebrazeh, M., Seyfizadeh, N., Aslani, S., Salimi, S., ... & Mohammadi, H. (2020). The roles of ERAP1 and ERAP2 in autoimmunity and cancer immunity: New insights and perspective. Molecular immunology, 121, 7-19.

Bufalieri, F., Infante, P., Bernardi, F., Caimano, M., Romania, P., Moretti, M., ... & Di Marcotullio, L. (2019). ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. Nature communications, 10(1), 1-15.

Compagnone, M., Cifaldi, L., & Fruci, D. (2019). Regulation of ERAP1 and ERAP2 genes and their disfunction in human cancer. Human immunology, 80(5), 318-324.

Admon, A. (2019). ERAP1 shapes just part of the immunopeptidome. Human immunology, 80(5), 296-301.

Yao, Y., Liu, N., Zhou, Z., & Shi, L. (2019). Influence of ERAP1 and ERAP2 gene polymorphisms on disease susceptibility in different populations. Human immunology, 80(5), 325-334.

Reeves, E., & James, E. (2018). The role of polymorphic ERAP1 in autoinflammatory disease. Bioscience reports, 38(4).

López de Castro, J. A. (2018). How ERAP1 and ERAP2 shape the peptidomes of disease-associated MHC-I proteins. Frontiers in Immunology, 9, 2463.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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