Sign in or Register   Sign in or Register
  |  

Mouse Anti-ERCC6L (AA 1-419) Recombinant Antibody (3F12-2B10) (CBMAB-E0525-FY)

This product is mouse antibody that recognizes ERCC6L. The antibody 3F12-2B10 can be used for immunoassay techniques such as: ELISA, WB.
See all ERCC6L antibodies
Published Data
Fig. 1 HeLa cells were transfected with control or PICH-specific siRNA oligo using protocols indicated on the left. Fixed cell were stained with anti-PICH (green) and anti-Plk1 (red) antibodies and counterstained with DAPI (blue).
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F12-2B10
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
ERCC6L full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: MEKSFATKNE AVQKETLQEG PKQEALQEDP LESFNYVLSK STKADIGPNL DQLKDDEILR HCNPWPIISI TNESQNAESN VSIIEIADDL SASHSALQDA QASEAKLEEE PSASSPQYAC DFNLFLEDSA DNRQNFSSQS LEHVEKENSL CGSAPNSRAG FVHSKTCLSW EFSEKDDEPE EVVVKAKIRS KARRIVSDGE DEDDSFKDTS SINPFNTSLF QFSSVKQFDA STPKNDISPP GRFFSSQIPS SVNKSMNSRR SLASRRSLIN MVLDHVEDME ERLDDSSEAK GPEDYPEEGV EESSGEASKY TEEDPSGETL SSENKSSWLM TSKPSALAQE TSLGAPEPLS GEQLVGSPQD KAAEATNDYE TLVKRGKELK ECGKIQEALN CLVKALDIKS ADPEVMLLTL SLYKQLNNN
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-419

Target

Full Name
ERCC Excision Repair 6 Like, Spindle Assembly Checkpoint Helicase
Introduction
This gene encodes a member of the SWItch/Sucrose Non-Fermentable (SWI/SNF2) family of proteins, and contains a SNF2-like ATPase domain and a PICH family domain. One distinguishing feature of this SWI/SNF protein family member is that during interphase, the protein is excluded from the nucleus, and only associates with chromatin after the nuclear envelope has broken down. This protein is a DNA translocase that is thought to bind double-stranded DNA that is exposed to stretching forces, such as those exerted by the mitotic spindle. This protein associates with ribosomal DNA and ultra-fine DNA bridges (UFBs), fine structures that connect sister chromatids during anaphase at some sites such as fragile sites, telomeres and centromeres. This gene is required for the faithful segregation of sister chromatids during mitosis, and the ATPase activity of this protein required for the resolution of UFBs before cytokinesis.
Entrez Gene ID
54821
UniProt ID
Q2NKX8
Alternative Names
ERCC Excision Repair 6 Like, Spindle Assembly Checkpoint Helicase; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like; Excision Repair Cross-Complementation Group 6 Like; PLK1-Interacting Checkpoint Helicase; ATP-Dependent Helicase ERCC6-Like; Tumor Antigen BJ-HCC-15; PICH; Excision Repair Cross-Complementing Rodent Repair Deficiency Complementation Group 6 - Like
Research Area
DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase (PubMed:17218258, PubMed:23973328).

Functions as ATP-dependent DNA translocase (PubMed:23973328, PubMed:28977671).

Can promote Holliday junction branch migration (in vitro) (PubMed:23973328).
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cellular Location
Centromere; Kinetochore; Chromosome. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
PTM
Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.

Huang, X., Jiang, L., Lu, S., Yuan, M., Lin, H., Li, B., ... & Zhong, Y. (2022). Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma. Oncology Reports, 48(1), 1-18.

Hou, G., Lu, Z., Bi, Y., Deng, J., & Yang, X. (2022). ERCC6L is a biomarker and therapeutic target for non–small cell lung adenocarcinoma. Medical Oncology, 39(5), 1-16.

Zhang, G., Yu, Z., Fu, S., Lv, C., Dong, Q., Fu, C., ... & Zeng, Y. (2022). Correction to: ERCC6L that is up-regulated in high grade of renal cell carcinoma enhances cell viability in vitro and promotes tumor growth in vivo potentially through modulating MAPK signalling pathway. Cancer Gene Therapy, 1-1.

Zhang, G., Ma, J., Xiong, J., Huang, X., Han, X., Yu, X., & Jiang, X. (2021). Upregulation of Excision Repair Cross-Complementation Group 6-Like (ERCC6L) Promotes Tumor Growth in Hepatocellular Carcinoma. Digestive Diseases and Sciences, 66(4), 1097-1109.

Chen, D., Liu, Q., & Cao, G. (2021). ERCC6L promotes cell growth and metastasis in gastric cancer through activating NF-κB signaling. Aging (Albany NY), 13(16), 20218.

Yu, B., Liang, H., Ye, Q., & Wang, Y. (2020). Upregulation of ERCC6L is associated with tumor progression and unfavorable prognosis in hepatocellular carcinoma. Journal of Gastrointestinal Oncology, 11(5), 1009.

Chen, H., Wang, H., Yu, X., Zhou, S., Zhang, Y., Wang, Z., ... & Wang, Z. (2020). ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway. BMC cancer, 20(1), 1-10.

Xie, Y., Yu, J., Wang, F., Li, M., Qiu, X., Liu, Y., & Qi, J. (2019). ERCC6L promotes cell growth and invasion in human colorectal cancer. Oncology letters, 18(1), 237-246.

Zhang, G., Yu, Z., Fu, S., Lv, C., Dong, Q., Fu, C., ... & Zeng, Y. (2019). ERCC6L that is up-regulated in high grade of renal cell carcinoma enhances cell viability in vitro and promotes tumor growth in vivo potentially through modulating MAPK signalling pathway. Cancer Gene Therapy, 26(9), 323-333.

Liu, J., Sun, J., Zhang, Q., & Zeng, Z. (2018). shRNA knockdown of DNA helicase ERCC6L expression inhibits human breast cancer growth. Molecular Medicine Reports, 18(3), 3490-3496.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ERCC6L (AA 1-419) Recombinant Antibody (3F12-2B10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare