Mouse Anti-FBXO4 Recombinant Antibody (CBXF-0411) (CBMAB-F0361-CQ)

Name: Mouse Anti-FBXO4 Recombinant Antibody (CBXF-0411)
SKU: CBMAB-F0361-CQ
Rating: 5 (1 reviews)

See all FBXO4 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0411

Application

ELISA, IF, IP

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

F-box protein 4

Introduction

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

26272

UniProt ID

Q9UKT5

Alternative Names

F-Box Protein 4; F-Box Only Protein 4; FBX4; F-Box Protein Fbx4;

Research Area

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1.

Biological Process

Aging Source: Ensembl
Cellular homeostasis Source: Ensembl
Cellular response to ionizing radiation Source: Ensembl
Common myeloid progenitor cell proliferation Source: Ensembl
Negative regulation of fibroblast proliferation Source: Ensembl
Negative regulation of protein localization to nucleus Source: Ensembl
Positive regulation of protein polyubiquitination Source: Ensembl
Positive regulation of protein ubiquitination Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Posttranscriptional regulation of gene expression Source: Ensembl
Protein destabilization Source: Ensembl
Protein polyubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of DNA damage checkpoint Source: Ensembl
Regulation of protein stability Source: BHF-UCL
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Telomere maintenance Source: UniProtKB
Ubiquitin-dependent protein catabolic process Source: UniProtKB

Cellular Location

Cytoplasm

PTM

Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1.

Qie, S. (2022). The E3 Ubiquitin Ligase Fbxo4 Functions as a Tumor Suppressor: Its Biological Importance and Therapeutic Perspectives. Cancers, 14(9), 2133.

Liu, H., Xue, Q., Cai, H., Jiang, X., Cao, G., Chen, T., ... & Wang, D. (2022). RUNX3-mediated circDYRK1A inhibits glutamine metabolism in gastric cancer by up-regulating microRNA-889-3p-dependent FBXO4. Journal of Translational Medicine, 20(1), 1-18.

Cao, H., Gao, R., Yu, C., Chen, L., & Feng, Y. (2019). The RNA-binding protein FXR1 modulates prostate cancer progression by regulating FBXO4. Functional & integrative genomics, 19(3), 487-496.

Dhar, R., Rath, G. K., & Karmakar, S. (2019). FBXO4 as a novel ubiquitin ligase that targets Cyclin D in the pathogenesis of breast cancer.

Peng, J., Li, Y., Wang, X., Deng, S., Holland, J., Yates, E., ... & Fan, G. C. (2018). An Hsp20-FBXO4 axis regulates adipocyte function through modulating PPARγ ubiquitination. Cell reports, 23(12), 3607-3620.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-FBXO4 Recombinant Antibody (CBXF-0411)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-FBXO4 Recombinant Antibody (2F2) (CAT#: CBMAB-F1955-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry