Rabbit Anti-FOXH1 Recombinant Antibody (EG1227) (CBMAB-EN1484-LY)

Name: Rabbit Anti-FOXH1 Recombinant Antibody (EG1227)
SKU: CBMAB-EN1484-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1227

Application

IF: 1:100~1:500 ELISA: 1:5000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human FOXH1.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

forkhead box H1

Introduction

FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT.

Entrez Gene ID

Human	8928
Mouse	14106

UniProt ID

Human	O75593
Mouse	O88621

Alternative Names

FAST1; FAST-1

Function

Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

Biological Process

Aorta morphogenesis Source: Ensembl
Axial mesoderm development Source: Ensembl
Cardiac right ventricle morphogenesis Source: Ensembl
Cellular response to cytokine stimulus Source: UniProtKB
Embryonic heart tube anterior/posterior pattern specification Source: Ensembl
Heart looping Source: Ensembl
Negative regulation of androgen receptor signaling pathway Source: BHF-UCL
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of intracellular estrogen receptor signaling pathway Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry Source: BHF-UCL
Outflow tract morphogenesis Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Secondary heart field specification Source: Ensembl
Transforming growth factor beta receptor signaling pathway Source: BHF-UCL
Ventricular trabecula myocardium morphogenesis Source: Ensembl

Cellular Location

Nucleus

Pluta, R., Aragón, E., Prescott, N. A., Ruiz, L., Mees, R. A., Baginski, B., ... & Macias, M. J. (2022). Molecular basis for DNA recognition by the maternal pioneer transcription factor FoxH1. Nature Communications, 13(1), 7279.

Zhou, J. J., Pham, P. D., Han, H., Wang, W., & Cho, K. W. (2022). Engagement of Foxh1 in chromatin regulation revealed by protein interactome analyses. Development, Growth & Differentiation, 64(6), 297-305.

Ouyang, X., Feng, L., Yao, L., Zhang, J., Xiao, Y., Liu, G., ... & Wang, Z. (2022). A comprehensive analysis of FOX family in HCC and experimental evidence to support the oncogenic role of FOXH1. Aging (Albany NY), 14(5), 2268.

Zhang, J., Zhang, X., Yang, S., Bao, Y., Xu, D., & Liu, L. (2021). FOXH1 promotes lung cancer progression by activating the Wnt/β-catenin signaling pathway. Cancer cell international, 21(1), 1-13.

Afouda, B. A., Nakamura, Y., Shaw, S., Charney, R. M., Paraiso, K. D., Blitz, I. L., ... & Hoppler, S. (2020). Foxh1/Nodal defines context-specific direct maternal Wnt/β-catenin target gene regulation in early development. Iscience, 23(7), 101314.

Tao, W., Shi, H., Yang, J., Diakite, H., Kocher, T. D., & Wang, D. (2020). Homozygous mutation of foxh1 arrests oogenesis causing infertility in female Nile tilapia. Biology of Reproduction, 102(3), 758-769.

Aragón, E., Wang, Q., Zou, Y., Morgani, S. M., Ruiz, L., Kaczmarska, Z., ... & Massagué, J. (2019). Structural basis for distinct roles of SMAD2 and SMAD3 in FOXH1 pioneer-directed TGF-β signaling. Genes & development, 33(21-22), 1506-1524.

Wang, L., Su, Y., Huang, C., Yin, Y., Zhu, J., Knupp, A., ... & Tang, Y. (2019). FOXH1 Is Regulated by NANOG and LIN28 for Early-stage Reprogramming. Scientific Reports, 9(1), 16443.

Amelio, I. (2019). How mutant p53 empowers Foxh1 fostering leukaemogenesis?. Cell Death Discovery, 5(1), 108.

Fischer, P., Chen, H., Pacho, F., Rieder, D., Kimmel, R. A., & Meyer, D. (2019). FoxH1 represses miR-430 during early embryonic development of zebrafish via non-canonical regulation. BMC biology, 17, 1-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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