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Mouse Anti-IL15 Recombinant Antibody (2H7) (CBMAB-A4354-LY)

The product is antibody recognizes IL15. The antibody 2H7 immunoassay techniques such as: IF, ELISA.
See all IL15 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2H7
Antibody Isotype
IgG1, κ
Application
IF, ELISA

Basic Information

Immunogen
IL15 (NP_000576, 49 a.a. ~ 162 a.a) full length recombinant protein.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Interleukin 15
Introduction
The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported. [provided by RefSeq]
Entrez Gene ID
UniProt ID
Alternative Names
IL-15; MGC9721
Function
Cytokine that stimulates the proliferation of T-lymphocytes (PubMed:8178155).

Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA (PubMed:8178155).

In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation (PubMed:15123770).
Biological Process
Aging Source: Ensembl
Cell-cell signaling Source: ProtInc
Cell maturation Source: Ensembl
Cellular response to vitamin D Source: Ensembl
Extrathymic T cell selection Source: Ensembl
Hyaluronan metabolic process Source: Ensembl
Immune response Source: ProtInc
Inflammatory response Source: Ensembl
Interleukin-15-mediated signaling pathway Source: UniProtKB
Lymph node development Source: Ensembl
Macrophage differentiation Source: ARUK-UCL
Negative regulation of cold-induced thermogenesis Source: YuBioLab
Negative regulation of smooth muscle cell proliferation Source: Ensembl
Neutrophil activation Source: UniProtKB
NK T cell proliferation Source: Ensembl
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of cytokine production Source: GO_Central
Positive regulation of immune response Source: GO_Central
Positive regulation of inflammatory response Source: BHF-UCL
Positive regulation of interleukin-17 production Source: BHF-UCL
Positive regulation of natural killer cell differentiation Source: Ensembl
Positive regulation of natural killer cell proliferation Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of phagocytosis Source: UniProtKB
Positive regulation of protein O-linked glycosylation Source: Ensembl
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of tissue remodeling Source: BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT protein Source: GO_Central
Regulation of defense response to virus by host Source: Ensembl
Regulation of T cell differentiation Source: Ensembl
Signal transduction Source: ProtInc
Skeletal muscle atrophy Source: Ensembl
Tyrosine phosphorylation of STAT protein Source: CACAO
Cellular Location
Isoform IL15-S48AA: Secreted
Isoform IL15-S21AA: Nucleus; Cytoplasm. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.

Aryee, K. E., Burzenski, L. M., Yao, L. C., Keck, J. G., Greiner, D. L., Shultz, L. D., & Brehm, M. A. (2022). Enhanced development of functional human NK cells in NOD‐scid‐IL2rgnull mice expressing human IL15. The FASEB Journal, 36(9).

Ma, R., Lu, T., Li, Z., Teng, K. Y., Mansour, A. G., Yu, M., ... & Yu, J. (2021). An oncolytic virus expressing IL15/IL15Rα combined with off-the-shelf EGFR-CAR NK cells targets glioblastoma. Cancer research, 81(13), 3635-3648.

Batra, S. A., Rathi, P., Guo, L., Courtney, A. N., Fleurence, J., Balzeau, J., ... & Heczey, A. (2020). Glypican-3–specific CAR T cells coexpressing IL15 and IL21 have superior expansion and antitumor activity against hepatocellular carcinoma. Cancer immunology research, 8(3), 309-320.

Chauvin, J. M., Ka, M., Pagliano, O., Menna, C., Ding, Q., DeBlasio, R., ... & Zarour, H. M. (2020). IL15 stimulation with TIGIT blockade reverses CD155-mediated NK-cell dysfunction in melanoma. Clinical Cancer Research, 26(20), 5520-5533.

Alizadeh, D., Wong, R. A., Yang, X., Wang, D., Pecoraro, J. R., Kuo, C. F., ... & Brown, C. E. (2019). IL15 enhances CAR-T cell antitumor activity by reducing mTORC1 activity and preserving their stem cell memory phenotype. Cancer immunology research, 7(5), 759-772.

Xu, X., Huang, W., Heczey, A., Liu, D., Guo, L., Wood, M., ... & Metelitsa, L. S. (2019). NKT cells coexpressing a GD2-specific chimeric antigen receptor and IL15 show enhanced in vivo persistence and antitumor activity against neuroblastoma. Clinical Cancer Research, 25(23), 7126-7138.

Conlon, K. C., Potter, E. L., Pittaluga, S., Lee, C. C. R., Miljkovic, M. D., Fleisher, T. A., ... & Waldmann, T. A. (2019). IL15 by continuous intravenous infusion to adult patients with solid tumors in a phase I trial induced dramatic NK-cell subset expansion. Clinical Cancer Research, 25(16), 4945-4954.

Margolin, K., Morishima, C., Velcheti, V., Miller, J. S., Lee, S. M., Silk, A. W., ... & Ernstoff, M. S. (2018). Phase I trial of ALT-803, a novel recombinant IL15 complex, in patients with advanced solid tumors. Clinical Cancer Research, 24(22), 5552-5561.

Miller, J. S., Morishima, C., McNeel, D. G., Patel, M. R., Kohrt, H. E., Thompson, J. A., ... & Conlon, K. C. (2018). A first-in-human phase I study of subcutaneous outpatient recombinant human IL15 (rhIL15) in adults with advanced solid tumors. Clinical Cancer Research, 24(7), 1525-1535.

Borrelli, C., Ricci, B., Vulpis, E., Fionda, C., Ricciardi, M. R., Petrucci, M. T., ... & Soriani, A. (2018). Drug-induced senescent multiple myeloma cells elicit NK cell proliferation by direct or exosome-mediated IL15 trans-presentation. Cancer immunology research, 6(7), 860-869.

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For research use only. Not intended for any clinical use.

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