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Mouse Anti-KDM6A Recombinant Antibody (CBT4129) (V2LY-0625-LY449)


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Summary

Host Animal
Mouse
Specificity
Human, Rat
Clone
CBT4129
Antibody Isotype
IgG2a
Application
WB

Basic Information

Immunogen
Purified recombinant fragment of human KDM6A (AA: 1252-1401) expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Lysine Demethylase 6A
Entrez Gene ID
7403
UniProt ID
O15550
Function
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17851529, PubMed:17713478, PubMed:17761849).
Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed:17851529, PubMed:17713478, PubMed:17761849).
Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).
Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed:17761849).
Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).
Biological Process
Chromatin remodelingISS:UniProtKB
Heart developmentManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-K27 demethylationManual Assertion Based On ExperimentIBA:GO_Central
Histone H3-K4 methylationBy SimilarityISS:UniProtKB
Regulation of gene expressionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Involvement in disease
Kabuki syndrome 2 (KABUK2):
A congenital mental retardation syndrome with additional features, including postnatal dwarfism, a peculiar facies characterized by long palpebral fissures with eversion of the lateral third of the lower eyelids, a broad and depressed nasal tip, large prominent earlobes, a cleft or high-arched palate, scoliosis, short fifth finger, persistence of fingerpads, radiographic abnormalities of the vertebrae, hands, and hip joints, and recurrent otitis media in infancy.
More Infomation

Duplaquet, L., Li, Y., Booker, M. A., Xie, Y., Olsen, S. N., Patel, R. A., ... & Oser, M. G. (2023). KDM6A epigenetically regulates subtype plasticity in small cell lung cancer. Nature Cell Biology, 25(9), 1346-1358.

Tran, N., Broun, A., & Ge, K. (2020). Lysine demethylase KDM6A in differentiation, development, and cancer. Molecular and cellular biology.

Stief, S. M., Hanneforth, A. L., Weser, S., Mattes, R., Carlet, M., Liu, W. H., ... & Spiekermann, K. (2020). Loss of KDM6A confers drug resistance in acute myeloid leukemia. Leukemia, 34(1), 50-62.

Gažová, I., Lengeling, A., & Summers, K. M. (2019). Lysine demethylases KDM6A and UTY: The X and Y of histone demethylation. Molecular genetics and metabolism, 127(1), 31-44.

Schulz, W. A., Lang, A., Koch, J., & Greife, A. (2019). The histone demethylase UTX/KDM6A in cancer: Progress and puzzles. International journal of cancer, 145(3), 614-620.

Chakraborty, A. A., Laukka, T., Myllykoski, M., Ringel, A. E., Booker, M. A., Tolstorukov, M. Y., ... & Kaelin Jr, W. G. (2019). Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Science, 363(6432), 1217-1222.

Itoh, Y., Golden, L. C., Itoh, N., Matsukawa, M. A., Ren, E., Tse, V., ... & Voskuhl, R. R. (2019). The X-linked histone demethylase Kdm6a in CD4+ T lymphocytes modulates autoimmunity. The Journal of clinical investigation, 129(9), 3852-3863.

Zhou, C., Wang, Y., Zhang, J., Su, J., An, Q., Liu, X., ... & Zhang, Y. (2019). H3K27me3 is an epigenetic barrier while KDM6A overexpression improves nuclear reprogramming efficiency. The FASEB Journal, 33(3), 4638-4652.

Cui, Y., Yan, H., Wang, K., Xu, H., Zhang, X., Zhu, H., ... & Pan, C. (2018). Insertion/deletion within the KDM6A gene is significantly associated with litter size in goat. Frontiers in Genetics, 9, 91.

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For research use only. Not intended for any clinical use.

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