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Mouse Anti-KHK Recombinant Antibody (3C4) (CBMAB-K0810-LY)

This product is antibody recognizes KHK. The antibody 3C4 immunoassay techniques such as: FC, IHC.
See all KHK antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3C4
Antibody Isotype
IgG1
Application
FC, IHC

Basic Information

Immunogen
KHK antibody was raised in mouse using a full length recombinant protein of human KHK (NP_000212) produced in HEK293T cells, as the immunogen
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.5 mg-1 mg/mL
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Ketohexokinase
Introduction
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Entrez Gene ID
3795
UniProt ID
P50053
Alternative Names
Ketohexokinase; Hepatic Fructokinase; Fructokinase; Testicular Tissue Protein Li 102; Ketohexokinase (Fructokinase); EC 2.7.1.3;
Function
Catalyzes the phosphorylation of the ketose sugar fructose to fructose-1-phosphate.
Biological Process
Fructose metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Regulation of glycogen metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Response to fructoseManual Assertion Based On ExperimentIBA:GO_Central
Response to glucoseManual Assertion Based On ExperimentIBA:GO_Central
Response to insulinManual Assertion Based On ExperimentIBA:GO_Central
Response to sucroseManual Assertion Based On ExperimentIBA:GO_Central
Response to zinc ionManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm; Cytosol; Extracellular exosome
Involvement in disease
Fructosuria (FRUCT):
Benign defect of intermediary metabolism.

Helsley, R. N., Park, S. H., Vekaria, H. J., Sullivan, P. G., Conroy, L. R., Sun, R. C., ... & Softic, S. (2023). Ketohexokinase-C regulates global protein acetylation to decrease carnitine palmitoyltransferase 1a-mediated fatty acid oxidation. Journal of Hepatology, 79(1), 25-42.

Tee, S. S., Kim, N., Cullen, Q., Eskandari, R., Mamakhanyan, A., Srouji, R. M., ... & Keshari, K. R. (2022). Ketohexokinase-mediated fructose metabolism is lost in hepatocellular carcinoma and can be leveraged for metabolic imaging. Science Advances, 8(14), eabm7985.

Shepherd, E. L., Saborano, R., Northall, E., Matsuda, K., Ogino, H., Yashiro, H., ... & Lalor, P. F. (2021). Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis. JHEP Reports, 3(2), 100217.

Kim, J., Kang, J., Kang, Y. L., Woo, J., Kim, Y., Huh, J., & Park, J. W. (2020). Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nature communications, 11(1), 5436.

Futatsugi, K., Smith, A. C., Tu, M., Raymer, B., Ahn, K., Coffey, S. B., ... & Magee, T. V. (2020). Discovery of PF-06835919: a potent inhibitor of ketohexokinase (KHK) for the treatment of metabolic disorders driven by the overconsumption of fructose. Journal of Medicinal Chemistry, 63(22), 13546-13560.

Hayasaki, T., Ishimoto, T., Doke, T., Hirayama, A., Soga, T., Furuhashi, K., ... & Kadomatsu, K. (2019). Fructose increases the activity of sodium hydrogen exchanger in renal proximal tubules that is dependent on ketohexokinase. The Journal of Nutritional Biochemistry, 71, 54-62.

Lanaspa, M. A., Andres-Hernando, A., Orlicky, D. J., Cicerchi, C., Jang, C., Li, N., ... & Tolan, D. R. (2018). Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice. The Journal of clinical investigation, 128(6), 2226-2238.

Gao, W., Li, N., Li, Z., Xu, J., & Su, C. (2018). Ketohexokinase is involved in fructose utilization and promotes tumor progression in glioma. Biochemical and biophysical research communications, 503(3), 1298-1306.

Miller, C. O., Yang, X., Lu, K., Cao, J., Herath, K., Rosahl, T. W., ... & Akiyama, T. E. (2018). Ketohexokinase knockout mice, a model for essential fructosuria, exhibit altered fructose metabolism and are protected from diet-induced metabolic defects. American Journal of Physiology-Endocrinology and Metabolism, 315(3), E386-E393.

Liu, D., Sterpka, J. A., Vatner, D. F., Bell, M., Murray, S., Bhanot, S., ... & Samuel, V. (2018). Targeting ketohexokinase (KHK) with a novel antisense oligonucleotide (ASO) decreases de novo lipogenesis and improves insulin-mediated whole body glucose metabolism. Diabetes, 67(Supplement_1).

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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