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Mouse Anti-MRGPRX2 Recombinant Antibody (CBT2365) (V2LY-0625-LY1829)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2365
Antibody Isotype
IgG1
Application
FC

Basic Information

Immunogen
Purified recombinant fragment of human MRGPRX2 expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MAS RELATED GPR FAMILY MEMBER X2
Entrez Gene ID
117194
UniProt ID
Q96LB1
Function
Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core (PubMed:25517090).

Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (By similarity).

Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.
Biological Process
G protein-coupled receptor signaling pathway Source: GO_Central
Mast cell activation Source: UniProtKB
Mast cell degranulation Source: UniProtKB
Positive regulation of cytokinesis Source: UniProtKB
Sensory perception of pain Source: UniProtKB
Sleep Source: UniProtKB
Cellular Location
Cell membrane
Topology
Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-63
Helical: 64-84
Extracellular: 85-96
Helical: 97-117
Cytoplasmic: 118-144
Helical: 145-165
Extracellular: 166-184
Helical: 185-205
Cytoplasmic: 206-228
Helical: 229-249
Extracellular: 250-264
Helical: 265-285
Cytoplasmic: 286-330
More Infomation

Zhang, F., Hong, F., Wang, L., Fu, R., Qi, J., & Yu, B. (2022). MrgprX2 regulates mast cell degranulation through PI3K/AKT and PLCγ signaling in pseudo-allergic reactions. International Immunopharmacology, 102, 108389.

Wolf, K., Kühn, H., Boehm, F., Gebhardt, L., Glaudo, M., Agelopoulos, K., ... & Kremer, A. E. (2021). A group of cationic amphiphilic drugs activates MRGPRX2 and induces scratching behavior in mice. Journal of Allergy and Clinical Immunology, 148(2), 506-522.

Ogasawara, H., & Noguchi, M. (2021). Therapeutic potential of MRGPRX2 inhibitors on mast cells. Cells, 10(11), 2906.

Deepak, V., Komarow, H. D., Alblaihess, A. A., Carter, M. C., Metcalfe, D. D., & Ali, H. (2021). Expression of MRGPRX2 in skin mast cells of patients with maculopapular cutaneous mastocytosis. The Journal of Allergy and Clinical Immunology: In Practice, 9(10), 3841-3843.

Corbière, A., Loste, A., & Gaudenzio, N. (2021). MRGPRX2 sensing of cationic compounds—A bridge between nociception and skin diseases?. Experimental Dermatology, 30(2), 193-200.

Babina, M. (2020). The pseudo-allergic/neurogenic route of mast cell activation via MRGPRX2: Discovery, functional programs, regulation, relevance to disease, and relation with allergic stimulation. Itch, 5(2), e32.

Chompunud Na Ayudhya, C., Roy, S., Thapaliya, M., & Ali, H. (2020). Roles of a mast cell–specific receptor MRGPRX2 in host defense and inflammation. Journal of Dental Research, 99(8), 882-890.

Wang, Z., & Babina, M. (2020). MRGPRX2 signals its importance in cutaneous mast cell biology: Does MRGPRX2 connect mast cells and atopic dermatitis?. Experimental Dermatology, 29(11), 1104-1111.

Hamamura-Yasuno, E., Iguchi, T., Kumagai, K., Tsuchiya, Y., & Mori, K. (2020). Identification of the dog orthologue of human MAS-related G protein coupled receptor X2 (MRGPRX2) essential for drug-induced pseudo-allergic reactions. Scientific Reports, 10(1), 16146.

Ogasawara, H., Furuno, M., Edamura, K., & Noguchi, M. (2019). Novel MRGPRX2 antagonists inhibit IgE-independent activation of human umbilical cord blood-derived mast cells. Journal of leukocyte biology, 106(5), 1069-1077.

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For research use only. Not intended for any clinical use.

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