Mouse Anti-MRGPRX2 Recombinant Antibody (1D3) (CBMAB-1606-CN)

Name: Mouse Anti-MRGPRX2 Recombinant Antibody (1D3)
SKU: CBMAB-1606-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D3

Application

FC, IHC-P

Immunogen

DNA vaccine

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

99% PBS, 0.5% BSA, pH 7.2

Preservative

0.1% Kathon

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MAS RELATED GPR FAMILY MEMBER X2

Introduction

Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core (PubMed:25517090). This protein acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.

Entrez Gene ID

117194

UniProt ID

Q96LB1

Alternative Names

MGRG3; MRGX2

Function

Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core (PubMed:25517090).

Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (By similarity).

Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.

Biological Process

G protein-coupled receptor signaling pathway Source: GO_Central
Mast cell activation Source: UniProtKB
Mast cell degranulation Source: UniProtKB
Positive regulation of cytokinesis Source: UniProtKB
Sensory perception of pain Source: UniProtKB
Sleep Source: UniProtKB

Cellular Location

Cell membrane

Topology

Extracellular: 1-33
Helical: 34-54
Cytoplasmic: 55-63
Helical: 64-84
Extracellular: 85-96
Helical: 97-117
Cytoplasmic: 118-144
Helical: 145-165
Extracellular: 166-184
Helical: 185-205
Cytoplasmic: 206-228
Helical: 229-249
Extracellular: 250-264
Helical: 265-285
Cytoplasmic: 286-330

Zhang, F., Hong, F., Wang, L., Fu, R., Qi, J., & Yu, B. (2022). MrgprX2 regulates mast cell degranulation through PI3K/AKT and PLCγ signaling in pseudo-allergic reactions. International Immunopharmacology, 102, 108389.

Wolf, K., Kühn, H., Boehm, F., Gebhardt, L., Glaudo, M., Agelopoulos, K., ... & Kremer, A. E. (2021). A group of cationic amphiphilic drugs activates MRGPRX2 and induces scratching behavior in mice. Journal of Allergy and Clinical Immunology, 148(2), 506-522.

Ogasawara, H., & Noguchi, M. (2021). Therapeutic potential of MRGPRX2 inhibitors on mast cells. Cells, 10(11), 2906.

Deepak, V., Komarow, H. D., Alblaihess, A. A., Carter, M. C., Metcalfe, D. D., & Ali, H. (2021). Expression of MRGPRX2 in skin mast cells of patients with maculopapular cutaneous mastocytosis. The Journal of Allergy and Clinical Immunology: In Practice, 9(10), 3841-3843.

Corbière, A., Loste, A., & Gaudenzio, N. (2021). MRGPRX2 sensing of cationic compounds—A bridge between nociception and skin diseases?. Experimental Dermatology, 30(2), 193-200.

Babina, M. (2020). The pseudo-allergic/neurogenic route of mast cell activation via MRGPRX2: Discovery, functional programs, regulation, relevance to disease, and relation with allergic stimulation. Itch, 5(2), e32.

Chompunud Na Ayudhya, C., Roy, S., Thapaliya, M., & Ali, H. (2020). Roles of a mast cell–specific receptor MRGPRX2 in host defense and inflammation. Journal of Dental Research, 99(8), 882-890.

Wang, Z., & Babina, M. (2020). MRGPRX2 signals its importance in cutaneous mast cell biology: Does MRGPRX2 connect mast cells and atopic dermatitis?. Experimental Dermatology, 29(11), 1104-1111.

Hamamura-Yasuno, E., Iguchi, T., Kumagai, K., Tsuchiya, Y., & Mori, K. (2020). Identification of the dog orthologue of human MAS-related G protein coupled receptor X2 (MRGPRX2) essential for drug-induced pseudo-allergic reactions. Scientific Reports, 10(1), 16146.

Ogasawara, H., Furuno, M., Edamura, K., & Noguchi, M. (2019). Novel MRGPRX2 antagonists inhibit IgE-independent activation of human umbilical cord blood-derived mast cells. Journal of leukocyte biology, 106(5), 1069-1077.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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