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Mouse Anti-P4HB Recombinant Antibody (CBT2429) (V2LY-0625-LY1849)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2429
Antibody Isotype
IgG2a
Application
WB, IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human P4HB (AA:309-508) expressed in mammalian.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Prolyl 4-Hydroxylase Subunit Beta
Entrez Gene ID
5034
UniProt ID
P07237
Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).
Biological Process
Cellular response to hypoxiaManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to interleukin-7IEA:Ensembl
Interleukin-12-mediated signaling pathwayTAS:Reactome
Interleukin-23-mediated signaling pathwayTAS:Reactome
Peptidyl-proline hydroxylation to 4-hydroxy-L-prolineManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell adhesionManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of viral entry into host cellManual Assertion Based On ExperimentIMP:UniProtKB
Protein foldingManual Assertion Based On ExperimentIBA:GO_Central
Protein folding in endoplasmic reticulumManual Assertion Based On ExperimentIDA:FlyBase
Regulation of oxidative stress-induced intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Response to endoplasmic reticulum stressManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular Location
Endoplasmic reticulum
Endoplasmic reticulum lumen
Melanosome
Cell membrane
Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces (PubMed:11181151).
Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:10636893).
Colocalizes with MTTP in the endoplasmic reticulum (PubMed:23475612).
Involvement in disease
Cole-Carpenter syndrome 1 (CLCRP1):
A form of Cole-Carpenter syndrome, a disorder characterized by features of osteogenesis imperfecta such as bone deformities and severe bone fragility with frequent fractures, in association with craniosynostosis, ocular proptosis, hydrocephalus, growth failure and distinctive facial features. Craniofacial findings include marked frontal bossing, midface hypoplasia, and micrognathia. Despite the craniosynostosis and hydrocephalus, intellectual development is normal. CLCRP1 inheritance is autosomal dominant.
More Infomation

Feng, D., Li, L., Li, D., Wu, R., Zhu, W., Wang, J., ... & Han, P. (2023). Prolyl 4-hydroxylase subunit beta (P4HB) could serve as a prognostic and radiosensitivity biomarker for prostate cancer patients. European Journal of Medical Research, 28(1), 245.

Wang, F., Yu, Z., Liu, X., Hu, F., Liu, X., Fu, X., ... & Zou, Z. (2023). A meta-analysis and bioinformatics analysis of p4hb expression levels in the prognosis of cancer patients. Pathology-Research and Practice, 154474.

Bai, F., Yu, K., Yang, Y., Zhang, Y., Ding, L., An, X., ... & Yang, X. (2022). Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy. Frontiers in Genetics, 13, 965816.

Wu, Y., Peng, Y., Guan, B., He, A., Yang, K., He, S., ... & Zhou, L. (2021). P4HB: A novel diagnostic and prognostic biomarker for bladder carcinoma. Oncology letters, 21(2), 1-1.

Yang, W., Wu, X., & Zhou, F. (2021). Collagen type X alpha 1 (COL10A1) contributes to cell proliferation, migration, and invasion by targeting prolyl 4-hydroxylase beta polypeptide (P4HB) in breast cancer. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 27, e928919-1.

Zhang, J., Guo, S., Wu, Y., Zheng, Z. C., Wang, Y., & Zhao, Y. (2019). P4HB, a novel hypoxia target gene related to gastric cancer invasion and metastasis. BioMed research international, 2019.

Zhu, Z., He, A., Lv, T., Xu, C., Lin, L., & Lin, J. (2019). Overexpression of P4HB is correlated with poor prognosis in human clear cell renal cell carcinoma. Cancer Biomarkers, 26(4), 431-439.

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For research use only. Not intended for any clinical use.

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