Mouse Anti-P4HB Recombinant Antibody (6-9H6) (CBMAB-P0616-YC)

Name: Mouse Anti-P4HB Recombinant Antibody (6-9H6)
SKU: CBMAB-P0616-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6-9H6

Application

ELISA, ICC, IF, IHC-Fr, IHC-P, WB

Specificity

Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2, 0.5% BSA, 0.09% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Prolyl 4-Hydroxylase Subunit Beta

Introduction

P4HB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Entrez Gene ID

18453

UniProt ID

P09103

Alternative Names

Prolyl 4-Hydroxylase Subunit Beta; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide; Protein Disulfide Isomerase Family A, Member 1; Protein Disulfide Isomerase-Associated 1; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase, Beta Polypeptide; Collagen Prolyl 4-Hydroxylase Beta; EC 5.3.4.1; ERBA2L; PO4DB; PDIA1; P55; PDI; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase; Thyroid Hormone Binding Protein P55); Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase-Associated 1);

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).

Biological Process

Cellular response to hypoxiaManual Assertion Based On ExperimentIMP:BHF-UCL
Cellular response to interleukin-7IEA:Ensembl
Interleukin-12-mediated signaling pathwayTAS:Reactome
Interleukin-23-mediated signaling pathwayTAS:Reactome
Peptidyl-proline hydroxylation to 4-hydroxy-L-prolineManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell adhesionManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:ARUK-UCL
Positive regulation of viral entry into host cellManual Assertion Based On ExperimentIMP:UniProtKB
Protein foldingManual Assertion Based On ExperimentIBA:GO_Central
Protein folding in endoplasmic reticulumManual Assertion Based On ExperimentIDA:FlyBase
Regulation of oxidative stress-induced intrinsic apoptotic signaling pathwayManual Assertion Based On ExperimentIMP:BHF-UCL
Response to endoplasmic reticulum stressManual Assertion Based On ExperimentIMP:BHF-UCL

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum lumen
Melanosome
Cell membrane
Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces (PubMed:11181151).
Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:10636893).
Colocalizes with MTTP in the endoplasmic reticulum (PubMed:23475612).

Involvement in disease

Cole-Carpenter syndrome 1 (CLCRP1):
A form of Cole-Carpenter syndrome, a disorder characterized by features of osteogenesis imperfecta such as bone deformities and severe bone fragility with frequent fractures, in association with craniosynostosis, ocular proptosis, hydrocephalus, growth failure and distinctive facial features. Craniofacial findings include marked frontal bossing, midface hypoplasia, and micrognathia. Despite the craniosynostosis and hydrocephalus, intellectual development is normal. CLCRP1 inheritance is autosomal dominant.

Feng, D., Li, L., Li, D., Wu, R., Zhu, W., Wang, J., ... & Han, P. (2023). Prolyl 4-hydroxylase subunit beta (P4HB) could serve as a prognostic and radiosensitivity biomarker for prostate cancer patients. European Journal of Medical Research, 28(1), 245.

Wang, F., Yu, Z., Liu, X., Hu, F., Liu, X., Fu, X., ... & Zou, Z. (2023). A meta-analysis and bioinformatics analysis of p4hb expression levels in the prognosis of cancer patients. Pathology-Research and Practice, 154474.

Bai, F., Yu, K., Yang, Y., Zhang, Y., Ding, L., An, X., ... & Yang, X. (2022). Identification and validation of P4HB as a novel autophagy-related biomarker in diabetic nephropathy. Frontiers in Genetics, 13, 965816.

Wu, Y., Peng, Y., Guan, B., He, A., Yang, K., He, S., ... & Zhou, L. (2021). P4HB: A novel diagnostic and prognostic biomarker for bladder carcinoma. Oncology letters, 21(2), 1-1.

Yang, W., Wu, X., & Zhou, F. (2021). Collagen type X alpha 1 (COL10A1) contributes to cell proliferation, migration, and invasion by targeting prolyl 4-hydroxylase beta polypeptide (P4HB) in breast cancer. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 27, e928919-1.

Zhang, J., Guo, S., Wu, Y., Zheng, Z. C., Wang, Y., & Zhao, Y. (2019). P4HB, a novel hypoxia target gene related to gastric cancer invasion and metastasis. BioMed research international, 2019.

Zhu, Z., He, A., Lv, T., Xu, C., Lin, L., & Lin, J. (2019). Overexpression of P4HB is correlated with poor prognosis in human clear cell renal cell carcinoma. Cancer Biomarkers, 26(4), 431-439.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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