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Rabbit Anti-PAXX Recombinant Antibody (D6X7X) (CBMAB-CP0055-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Rat
Clone
D6X7X
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro195 of human PAXX protein.
Specificity
Human, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
PAXX, Non-Homologous End Joining Factor
Introduction
The protein encoded by this gene plays a role in the nonhomologous end joining (NHEJ) pathway of DNA double-strand break repair. The encoded protein may function to stabilize the Ku70/Ku80 heterodimer to facilitate the assembly and maintain the stability of the NHEJ complex. [provided by RefSeq, Jul 2016]
Entrez Gene ID
Human286257
Rat296565
UniProt ID
HumanQ9BUH6
RatA0A0G2QC22
Alternative Names
PAXX; Non-Homologous End Joining Factor; Paralog Of XRCC4 And XLF; XRCC4-Like Small Protein; C9orf142; XLS; Chromosome 9 Open Reading Frame 142; Protein PAXX;
Function
Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ); participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:27705800).
May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:25574025, PubMed:25670504, PubMed:25941166).
Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (PubMed:27703001).
Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed:30250067, PubMed:25670504).
Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27705800).
Able to restrict infection by herpesvirus 1 (HSV-1) via an unknown mechanism (PubMed:29144403).
Biological Process
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Chromosome
Predominantly localizes to the nucleus. Accumulates at sites of DNA damage generated by laser microirradiation.2 Publications
Cytoplasm
(Microbial infection) Upon infection by herpesvirus 1 (HSV-1), it is partially translocated into the cytoplasm in an HSV-1-dependent manner.
PTM
Phosphorylation may inhibit interaction with the DNA-bound XRCC5/Ku80 and XRCC6/Ku70 heterodimer (Ku complex).
More Infomation

Khan, H., & Ochi, T. (2023). Plant PAXX has an XLF‐like function and stimulates DNA end joining by the Ku‐DNA ligase IV/XRCC4 complex. The Plant Journal, 116(1), 58-68.

Vogt, A., & He, Y. (2023). Structure and mechanism in non-homologous end joining. DNA repair, 130, 103547.

Chaplin, A. K., & Blundell, T. L. (2020). Structural biology of multicomponent assemblies in DNA double-strand-break repair through non-homologous end joining. Current opinion in structural biology, 61, 9-16.

Castañeda‐Zegarra, S., Fernandez‐Berrocal, M., Tkachov, M., Yao, R., Upfold, N. L. E., & Oksenych, V. (2020). Genetic interaction between the non‐homologous end‐joining factors during B and T lymphocyte development: In vivo mouse models. Scandinavian Journal of Immunology, 92(4), e12936.

Gago-Fuentes, R., & Oksenych, V. (2020). Non-homologous end joining factors xlf, paxx and dna-pkcs maintain the neural stem and progenitor cell population. Biomolecules, 11(1), 20.

Castañeda-Zegarra, S., Zhang, Q., Alirezaylavasani, A., Fernandez-Berrocal, M., Yao, R., & Oksenych, V. (2020). Leaky severe combined immunodeficiency in mice lacking non-homologous end joining factors XLF and MRI. Aging (Albany NY), 12(23), 23578.

Alirezaylavasani, A. (2020). Genetic interaction between accessory non-homologous end joining factors in B and T lymphocyte development (Master's thesis, NTNU).

Trigg, B. J. (2019). An exploration of the interplay between HSV-1 and the non-homologous end joining proteins PAXX and DNA-PKcs (Doctoral dissertation).

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For research use only. Not intended for any clinical use.

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