Mouse Anti-PAXX Recombinant Antibody (CBWJC-4269) (CBMAB-C5626WJ)

Name: Mouse Anti-PAXX Recombinant Antibody (CBWJC-4269)
SKU: CBMAB-C5626WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-4269

Application

WB, IP, IF, ELISA

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

PAXX, Non-Homologous End Joining Factor

Introduction

The protein encoded by this gene plays a role in the nonhomologous end joining (NHEJ) pathway of DNA double-strand break repair. The encoded protein may function to stabilize the Ku70/Ku80 heterodimer to facilitate the assembly and maintain the stability of the NHEJ complex. [provided by RefSeq, Jul 2016]

Entrez Gene ID

286257

UniProt ID

Q9BUH6

Alternative Names

PAXX, Non-Homologous End Joining Factor; Paralog Of XRCC4 And XLF; XRCC4-Like Small Protein; C9orf142; XLS; Chromosome 9 Open Reading Frame 142; Protein PAXX;

Function

Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ); participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:27705800).
May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:25574025, PubMed:25670504, PubMed:25941166).
Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (PubMed:27703001).
Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed:30250067, PubMed:25670504).
Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27705800).
Able to restrict infection by herpesvirus 1 (HSV-1) via an unknown mechanism (PubMed:29144403).

Biological Process

Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
Double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus
Chromosome
Predominantly localizes to the nucleus. Accumulates at sites of DNA damage generated by laser microirradiation.2 Publications
Cytoplasm
(Microbial infection) Upon infection by herpesvirus 1 (HSV-1), it is partially translocated into the cytoplasm in an HSV-1-dependent manner.

PTM

Phosphorylation may inhibit interaction with the DNA-bound XRCC5/Ku80 and XRCC6/Ku70 heterodimer (Ku complex).

Khan, H., & Ochi, T. (2023). Plant PAXX has an XLF‐like function and stimulates DNA end joining by the Ku‐DNA ligase IV/XRCC4 complex. The Plant Journal, 116(1), 58-68.

Vogt, A., & He, Y. (2023). Structure and mechanism in non-homologous end joining. DNA repair, 130, 103547.

Chaplin, A. K., & Blundell, T. L. (2020). Structural biology of multicomponent assemblies in DNA double-strand-break repair through non-homologous end joining. Current opinion in structural biology, 61, 9-16.

Castañeda‐Zegarra, S., Fernandez‐Berrocal, M., Tkachov, M., Yao, R., Upfold, N. L. E., & Oksenych, V. (2020). Genetic interaction between the non‐homologous end‐joining factors during B and T lymphocyte development: In vivo mouse models. Scandinavian Journal of Immunology, 92(4), e12936.

Gago-Fuentes, R., & Oksenych, V. (2020). Non-homologous end joining factors xlf, paxx and dna-pkcs maintain the neural stem and progenitor cell population. Biomolecules, 11(1), 20.

Castañeda-Zegarra, S., Zhang, Q., Alirezaylavasani, A., Fernandez-Berrocal, M., Yao, R., & Oksenych, V. (2020). Leaky severe combined immunodeficiency in mice lacking non-homologous end joining factors XLF and MRI. Aging (Albany NY), 12(23), 23578.

Alirezaylavasani, A. (2020). Genetic interaction between accessory non-homologous end joining factors in B and T lymphocyte development (Master's thesis, NTNU).

Trigg, B. J. (2019). An exploration of the interplay between HSV-1 and the non-homologous end joining proteins PAXX and DNA-PKcs (Doctoral dissertation).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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