NCBP1

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008]

NCBP1

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).

7-methylguanosine mRNA capping Source: UniProtKB
Alternative mRNA splicing, via spliceosome Source: ComplexPortal
Cap-dependent translational initiation Source: ComplexPortal
Defense response to virus Source: ComplexPortal
Gene silencing by miRNA Source: ComplexPortal
Histone mRNA metabolic process Source: ComplexPortal
mRNA 3'-end processing Source: ComplexPortal
mRNA export from nucleus Source: UniProtKB
mRNA splicing, via spliceosome Source: ComplexPortal
mRNA transcription by RNA polymerase II Source: ComplexPortal
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Positive regulation of cell growth Source: UniProtKB
Positive regulation of mRNA 3'-end processing Source: UniProtKB
Positive regulation of mRNA splicing, via spliceosome Source: CAFA
Positive regulation of phosphorylation of RNA polymerase II C-terminal domain Source: ComplexPortal
Positive regulation of RNA binding Source: CAFA
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Pre-mRNA cleavage required for polyadenylation Source: UniProtKB
Primary miRNA processing Source: ComplexPortal
Regulation of mRNA processing Source: GO_Central
Regulation of translational initiation Source: UniProtKB
RNA catabolic process Source: UniProtKB
RNA interference Source: ComplexPortal
RNA splicing Source: ProtInc
snRNA export from nucleus Source: ComplexPortal
Spliceosomal complex assembly Source: CAFA

Cytoplasm
Nucleus
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.