Mouse Anti-CTNND1 Recombinant Antibody (CBFYC-2414) (CBMAB-C2487-FY)

Name: Mouse Anti-CTNND1 Recombinant Antibody (CBFYC-2414)
SKU: CBMAB-C2487-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CTNND1 Recombinant Antibody (CBFYC-2414) (CBMAB-C2487-FY) in WB

Western blot showing the inhibition of p120ctn Ser320 phosphorylation by PF-3758309 U2OS cells. ...View More

Baskaran, Y., Tay, F. P. L., Ng, E. Y. W., Swa, C. L. F., Wee, S., Gunaratne, J., & Manser, E. (2021). Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions. Nature Communications, 12(1), 5315. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTNND1 Recombinant Antibody (CBFYC-2414) (CBMAB-C2487-FY) in IF

Fluorescent immunostaining. The purple region indicates the p120 expression present in vascular organoids. Scale bar, 100 µm. ...View More

Yanagisawa, K., Konno, M., Liu, H., Irie, S., Mizushima, T., Mori, M., ... & Ishii, H. (2020). A four-dimensional organoid system to visualize cancer cell vascular invasion. Biology, 9(11), 361. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTNND1 Recombinant Antibody (CBFYC-2414) (CBMAB-C2487-FY) in IP

P120 isoform association with adherens junctions in 4T1 cells. (A) Membrane-cytosol fractionation. (B) Co-immunoprecipitation with isoform-specific antibodies for p120-1 (6H11 mAb) and p120-3 (anti-p120-3 pAb). IP antibodies are indicated above the blots (* p < 0.05). (C) Cytosol extraction. All membranes were incubated with the antibodies indicated adjacent to the blots. In the blots stained for p120, the upper band represents p120-1 and the lower band represents p120-3. Quantifications are shown in bar graphs below the blots. ...View More

Venhuizen, J. H., Span, P. N., van den Dries, K., Sommer, S., Friedl, P., & Zegers, M. M. (2019). P120 catenin isoforms differentially associate with breast cancer invasion and metastasis. Cancers, 11(10), 1459. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CTNND1 Recombinant Antibody (CBFYC-2414) (CBMAB-C2487-FY) in IF

Confluent 4T1 cells show heterogeneous expression of p120 isoforms upon immunostaining with p120-3 (anti-p120-3 pAb) and p120-1 (6H11 mAb) and homogeneous expression of total p120 (anti-p120 mAb) and E-cadherin (DECMA-1 mAb). ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-2414

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 8-349 of mouse p120.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

More Infomation

Target

Full Name

Catenin Delta 1

Entrez Gene ID

Human	1500
Mouse	12388
Rat	311163

UniProt ID

Human	O60716
Mouse	P30999
Rat	D3ZZZ9

Function

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability (PubMed:14610055, PubMed:20371349).

Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics (PubMed:10207085, PubMed:20371349).

Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors (PubMed:17344476).

Biological Process

Adherens junction organization Source: Reactome
Brain development Source: Ensembl
Cell-cell adhesion Source: GO_Central
Cell-cell adhesion mediated by cadherin Source: UniProtKB
Cell-cell junction assembly Source: GO_Central
Entry of bacterium into host cell Source: Reactome
Negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
Protein stabilization Source: UniProtKB
Regulation of postsynaptic membrane neurotransmitter receptor levels Source: SynGO
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Nucleus; Cell membrane; Cytoplasm; Adherens junction. Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187).
Isoform 1A&2A&3A: Nucleus

Involvement in disease

Blepharocheilodontic syndrome 2 (BCDS2):
A form of blepharocheilodontic syndrome, a rare autosomal dominant disorder. It is characterized by lower eyelid ectropion, upper eyelid distichiasis, euryblepharon, bilateral cleft lip and palate, and features of ectodermal dysplasia, including hair anomalies, conical teeth and tooth agenesis. An additional rare manifestation is imperforate anus. There is considerable phenotypic variability among affected individuals.

PTM

Phosphorylated by FER and other protein-tyrosine kinases. Phosphorylated at Ser-288 by PAK5. Dephosphorylated by PTPRJ.

Liu, Y., Yuan, H., & He, T. (2022). Downregulated circular RNA hsa_circ_0005797 inhibits endometrial cancer by modulating microRNA-298/Catenin delta 1 signaling. Bioengineered, 13(3), 4634-4645.

Wei, D., Tian, M., Fan, W., Zhong, X., Wang, S., Chen, Y., & Zhang, S. (2021). Circular RNA circ_0000043 promotes endometrial carcinoma progression by regulating miR-1271-5p/CTNND1 axis. Archives of Gynecology and Obstetrics, 303(4), 1075-1087.

Gao, X. H., Zhang, Y. L., Zhang, Z. Y., Guo, S. S., Chen, X. B., & Guo, Y. Z. (2020). MicroRNA-96-5p represses breast cancer proliferation and invasion through Wnt/β-catenin signaling via targeting CTNND1. Scientific Reports, 10(1), 1-9.

Liu, D., Zhang, H., Cui, M., Chen, C., & Feng, Y. (2020). Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer. Cell Cycle, 19(15), 1917-1927.

Yu, X., Sheng, P., Sun, J., Zhao, X., Zhang, J., Li, Y., ... & Jiang, H. (2020). The circular RNA circMAST1 promotes hepatocellular carcinoma cell proliferation and migration by sponging miR-1299 and regulating CTNND1 expression. Cell death & disease, 11(5), 1-16.

Han, L., Li, Z., Jiang, Y., Jiang, Z., & Tang, L. (2019). SNHG29 regulates miR-223-3p/CTNND1 axis to promote glioblastoma progression via Wnt/β-catenin signaling pathway. Cancer cell international, 19(1), 1-11.

Shen, Q., He, T., & Yuan, H. (2019). Hsa_circ_0002577 promotes endometrial carcinoma progression via regulating miR-197/CTNND1 axis and activating Wnt/β-catenin pathway. Cell Cycle, 18(11), 1229-1240.

Cao, N., Mu, L., Yang, W., Liu, L., Liang, L., & Zhang, H. (2018). MicroRNA-298 represses hepatocellular carcinoma progression by inhibiting CTNND1-mediated Wnt/β-catenin signaling. Biomedicine & Pharmacotherapy, 106, 483-490.

Kievit, A., Tessadori, F., Douben, H., Jordens, I., Maurice, M., Hoogeboom, J., ... & van Haaften, G. (2018). Variants in members of the cadherin–catenin complex, CDH1 and CTNND1, cause blepharocheilodontic syndrome. European Journal of Human Genetics, 26(2), 210-219.

Liao, X. H., Zhang, Y., Dong, W. J., Shao, Z. M., & Li, D. Q. (2017). Chromatin remodeling protein MORC2 promotes breast cancer invasion and metastasis through a PRD domain-mediated interaction with CTNND1. Oncotarget, 8(58), 97941.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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