Mouse Anti-ALPL Antibody (B4-78) (CBMAB-1009CQ)

Name: Mouse Anti-ALPL Antibody (B4-78)
SKU: CBMAB-1009CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALPL Antibody (B4-78) in IF

Rhodamine-phalloidin staining and immunofluorescence of alkaline phosphatase (ALP) and osteocalcin (OC) in 2D-cultured MG-63 cells for 4 weeks with (a-c) proliferation medium or with (d-f) commercial MesenCult or (g-i) BMP2-supplemented osteogenic differentiation media. Cell nuclei were stained with DAPI and observed in blue, the distribution of actin filaments in red, and the presence of ALK or OC in green. ...View More

Martin-Iglesias, S., Milian, L., Sancho-Tello, M., Salvador-Clavell, R., Martín de Llano, J. J., Carda, C., & Mata, M. (2022). Bmp-2 enhances osteogenic differentiation of human adipose-derived and dental pulp stem cells in 2D and 3D in vitro models. Stem Cells International, 2022.

Mouse Anti-ALPL Antibody (B4-78) in FC

Isolation and characterization of interstitial stem cells from murine skeletal muscle. Gating strategy for FACS (fluorescent activated cell sorting) isolation of murine MABs (mesoangioblasts) and FAPs (fibro/adipogenic progenitors) (A) and control gates (B). ...View More

Camps, J., Grosemans, H., Gijsbers, R., Maes, C., & Sampaolesi, M. (2019). Growth factor screening in dystrophic muscles reveals PDGFB/PDGFRB-mediated migration of interstitial stem cells. International Journal of Molecular Sciences, 20(5), 1118.

Mouse Anti-ALPL Antibody (B4-78) in ICC

Immunocytochemistry of pluripotency markers.
Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc immortalized lines (hNS1, hVM1, and hCTX) and fibroblasts (hFF-1). From top to bottom the markers studied were: NANOG, OCT3/4, SOX2 and AP. Hoechst 33258 nuclear staining is shown in blue. Scale bar represents 100 μm for hESCs in the left hand column (low magnification) and 25 μm for all the other microphotographs. ...View More

Pino-Barrio, M. J., Garcia-Garcia, E., Menendez, P., & Martinez-Serrano, A. (2015). V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS One, 10(3), e0118499.

Mouse Anti-ALPL Antibody (B4-78) in FC

Expression of ALP and HLA-G5 was evaluated by intracellular flow cytometry analysis on AT-derived MSCs committed to 14-day osteodifferentiation process and pretreated with IFNγ and TNFα (Os-MSC-AT IFNγ/TNFα) or not (Os-MSC-AT). IgG1 was used as isotype control Ab. ...View More

Montespan, F., Deschaseaux, F., Sensébé, L., Carosella, E. D., & Rouas-Freiss, N. (2014). Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy. Journal of immunology research, 2014.

Mouse Anti-ALPL Antibody (B4-78) in IHC

Immunohistochemical analysis of DFC sheets. After DFCs were co-cultured for 3 weeks, the cells of the experimental sheets were found to be immuno-positive for osteoblast/cementoblast-related proteins, including ALP (A), OCN (C), BSP (E) and OPG (G), versus controls, which were immuno-negative for these proteins (B, D, F and H). Bar: 50 μm. ...View More

Bai, Y., Bai, Y., Matsuzaka, K., Hashimoto, S., Fukuyama, T., Wu, L., ... & Inoue, T. (2011). Cementum-and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells. Bone, 48(6), 1417-1426.

Mouse Anti-ALPL Antibody (B4-78) in ICC

Expression pattern of various osteogenic markers in tubular scaffold by confocal microscopy. Immunostaining of osteogenic tube co-cultures revealed areasmof cellular clustering and aggregation of mature osteoblasts represented by their distinct morphology and phenotypic expression (AeH). Localization of key osteoblastic phenotypic markers of day 9 osteoinductive co-culture tube sections demonstrated the expression of osteopontin (fuchsia, A,B), osteocalcin (red, C,D), osteonectin (fuchsia, E,F), and alkaline phosphatase (red, G,H). Cells were also stained for nuclei (blue, DAPI) and fibrillar actin (green, Alexa 488 phalloidin). Merged images AeH (AeH, scale bar 20 mm). ...View More

Valarmathi, M. T., Yost, M. J., Goodwin, R. L., & Potts, J. D. (2008). The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold. Biomaterials, 29(14), 2203-2216.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

B4-78

Application

FC, IF, IHC, WB

Immunogen

Purified human bone alkaline phosphatase

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	8-25 µg/ml
IF(ICC)	8-25 µg/ml
FC	0.25 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

ABS

Preservative

0.09% sodium azide

Concentration

0.5 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alkaline Phosphatase, Liver/Bone/Kidney

Introduction

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. Diseases associated with ALPL include Hypophosphatasia, Infantile and Hypophosphatasia, Childhood. Among its related pathways are AGE/RAGE pathway and Thiamine metabolism.

Entrez Gene ID

Human	249
Mouse	11647
Rat	25586

UniProt ID

Human	P05186
Mouse	P09242
Rat	P08289

Alternative Names

Alkaline Phosphatase; Liver/Bone/Kidney; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; EC 3.1.3.1; AP-TNAP; TNSALP; Liver/Bone/Kidney-Type Alkaline Phosphatase; Alkaline Phosphomonoesterase; Tissue-Nonspecific ALP; Glycerophosphatase; APTNAP; TNAP; HOPS

Function

This isozyme plays a key role in skeletal mineralization by regulating levels of diphosphate (PPi).

Biological Process

Cellular response to organic cyclic compound Source: Ensembl
Cementum mineralization Source: Ensembl
Dephosphorylation Source: GO_Central
Developmental process involved in reproduction Source: Ensembl
Endochondral ossification Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Response to antibiotic Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Response to vitamin D Source: BHF-UCL
Skeletal system development Source: ProtInc

Cellular Location

Cell membrane

Involvement in disease

Hypophosphatasia (HOPS): A metabolic bone disease characterized by defective skeletal mineralization and biochemically by deficient activity of the tissue non-specific isoenzyme of alkaline phosphatase. Four forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. The adult form is mild and characterized by recurrent fractures, osteomalacia, rickets, and loss of teeth. Some cases are asymptomatic, while some patients manifest dental features without skeletal manifestations (odontohypophosphatasia).
Hypophosphatasia childhood type (HOPSC): A bone disease characterized by defective skeletal mineralization and biochemically by deficient activity of the tissue non-specific isoenzyme of alkaline phosphatase.
Hypophosphatasia infantile type (HOPSI): A severe bone disease characterized by defective skeletal mineralization and biochemically by deficient activity of the tissue non-specific isoenzyme of alkaline phosphatase. Three more or less distinct types of infantile hypophosphatasia can be identified: (1) type 1 with onset in utero or in early postnatal life, craniostenosis, severe skeletal abnormalities, hypercalcemia, and death in the first year or so of life; (2) type 2 with later, more gradual development of symptoms, moderately severe 'rachitic' skeletal changes and premature loss of teeth; (3) type 3 with no symptoms, the condition being determined on routine studies.

PTM

N-glycosylated.

Vimalraj, S. (2020). Alkaline phosphatase: structure, expression and its function in bone mineralization. Gene, 754, 144855.

Pietraszek, A., Ledwójcik, G., Lewandowska-Łańcucka, J., Horak, W., Lach, R., Łatkiewicz, A., & Karewicz, A. (2020). Bioactive hydrogel scaffolds reinforced with alkaline-phosphatase containing halloysite nanotubes for bone repair applications. International Journal of Biological Macromolecules, 163, 1187-1195.

Haarhaus, M., Gilham, D., Kulikowski, E., Magnusson, P., & Kalantar-Zadeh, K. (2020). Pharmacologic epigenetic modulators of alkaline phosphatase in chronic kidney disease. Current opinion in nephrology and hypertension, 29(1), 4.

Li, N., Zhou, L., Xie, W., Zeng, D., Cai, D., Wang, H., ... & Li, L. (2019). Alkaline phosphatase enzyme-induced biomineralization of chitosan scaffolds with enhanced osteogenesis for bone tissue engineering. Chemical Engineering Journal, 371, 618-630.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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