Mouse Anti-ALPL Recombinant Antibody (3A7) (CBMAB-A0315-LY)

Name: Mouse Anti-ALPL Recombinant Antibody (3A7)
SKU: CBMAB-A0315-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A7

Application

WB, ELISA

Immunogen

ALPL (AAH21289, 28 a.a. ~ 137 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Alkaline Phosphatase, Liver/Bone/Kidney

Introduction

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

Entrez Gene ID

249

Alternative Names

AP-TNAP; FLJ40094; FLJ93059; HOPS; MGC161443; MGC167935; TNAP; TNSALP

Function

This isozyme plays a key role in skeletal mineralization by regulating levels of diphosphate (PPi).

Biological Process

Cellular response to organic cyclic compound Source: Ensembl
Cementum mineralization Source: Ensembl
Dephosphorylation Source: GO_Central
Developmental process involved in reproduction Source: Ensembl
Endochondral ossification Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Response to antibiotic Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Response to vitamin D Source: BHF-UCL
Skeletal system development Source: ProtInc

Cellular Location

Cell membrane

Involvement in disease

Hypophosphatasia (HOPS): A metabolic bone disease characterized by defective skeletal mineralization and biochemically by deficient activity of the tissue non-specific isoenzyme of alkaline phosphatase. Four forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. The adult form is mild and characterized by recurrent fractures, osteomalacia, rickets, and loss of teeth. Some cases are asymptomatic, while some patients manifest dental features without skeletal manifestations (odontohypophosphatasia).
Hypophosphatasia childhood type (HOPSC): A bone disease characterized by defective skeletal mineralization and biochemically by deficient activity of the tissue non-specific isoenzyme of alkaline phosphatase.
Hypophosphatasia infantile type (HOPSI): A severe bone disease characterized by defective skeletal mineralization and biochemically by deficient activity of the tissue non-specific isoenzyme of alkaline phosphatase. Three more or less distinct types of infantile hypophosphatasia can be identified: (1) type 1 with onset in utero or in early postnatal life, craniostenosis, severe skeletal abnormalities, hypercalcemia, and death in the first year or so of life; (2) type 2 with later, more gradual development of symptoms, moderately severe 'rachitic' skeletal changes and premature loss of teeth; (3) type 3 with no symptoms, the condition being determined on routine studies.

PTM

N-glycosylated.

Vimalraj, S. (2020). Alkaline phosphatase: structure, expression and its function in bone mineralization. Gene, 754, 144855.

Pietraszek, A., Ledwójcik, G., Lewandowska-Łańcucka, J., Horak, W., Lach, R., Łatkiewicz, A., & Karewicz, A. (2020). Bioactive hydrogel scaffolds reinforced with alkaline-phosphatase containing halloysite nanotubes for bone repair applications. International Journal of Biological Macromolecules, 163, 1187-1195.

Haarhaus, M., Gilham, D., Kulikowski, E., Magnusson, P., & Kalantar-Zadeh, K. (2020). Pharmacologic epigenetic modulators of alkaline phosphatase in chronic kidney disease. Current opinion in nephrology and hypertension, 29(1), 4.

Li, N., Zhou, L., Xie, W., Zeng, D., Cai, D., Wang, H., ... & Li, L. (2019). Alkaline phosphatase enzyme-induced biomineralization of chitosan scaffolds with enhanced osteogenesis for bone tissue engineering. Chemical Engineering Journal, 371, 618-630.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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