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Mouse Anti-ALX1 Recombinant Antibody (96k) (CBMAB-C0616-FY)

This product is mouse antibody that recognizes ALX1. The antibody CBFYC-0563 can be used for immunoassay techniques such as: WB.
See all ALX1 antibodies
Published Data
Mouse Anti-ALX1 Recombinant Antibody (96k) in IF
ALX1 is sparsely expressed early and diminishes later. Immunostaining for ALX1 (cyan) shows sparse expression in PAX6 (green) positive cells; it disappears from the cortex but is expressed in surrounding brain structures at CS22. ALX1 expression does begin until week 10 in the 13,234 cerebral organoids. For all panels, each sample was immunostained once. Scale bars, 50 μM. ...View More
Eze, U. C., Bhaduri, A., Haeussler, M., Nowakowski, T. J., & Kriegstein, A. R. (2021). Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Nature neuroscience, 24(4), 584-594.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
96k
Antibody Isotype
IgG2a, κ
Application
IP, IF, WB

Basic Information

Immunogen
Recombinant ALX1 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 198-307

Target

Full Name
ALX HOMEOBOX 1
Introduction
The specific function of this gene has yet to be determined in humans; however, in rodents, it is necessary for survival of the forebrain mesenchyme and may also be involved in development of the cervix. Mutations in the mouse gene lead to neural tube defects such as acrania and meroanencephaly.
Entrez Gene ID
8092
UniProt ID
Q15699
Alternative Names
ALX Homeobox 1; Cartilage Paired-Class Homeoprotein 1; CART-1; CART1; Epididymis Luminal Protein 23; Cartilage Homeoprotein 1; ALX Homeobox Protein 1; HEL23; FND3
Function
Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes (PubMed:9753625, PubMed:8756334). Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures. Early on in development, it plays a role in forebrain mesenchyme survival (PubMed:20451171). May also induce epithelial to mesenchymal transition (EMT) through the expression of SNAI1 (PubMed:23288509).
Biological Process
Embryonic skeletal system morphogenesis Source: GO_Central
Negative regulation of transcription, DNA-templated Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: MGI
Neural crest cell migration Source: InterPro
Positive regulation of epithelial to mesenchymal transition Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
Frontonasal dysplasia 3 (FND3): The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline.
PTM
Acetylated at Lys-131 by EP300; increases interaction with EP300 and stimulates ALX1 transcriptional activity.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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