Mouse Anti-ALX1 Recombinant Antibody (2A10) (CBMAB-A2468-YC)

Name: Mouse Anti-ALX1 Recombinant Antibody (2A10)
SKU: CBMAB-A2468-YC
Rating: 5 (1 reviews)

See all ALX1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A10

Application

ELISA, IF, WB

Immunogen

CART1 aa 198-306 partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ALX HOMEOBOX 1

Introduction

The specific function of this gene has yet to be determined in humans; however, in rodents, it is necessary for survival of the forebrain mesenchyme and may also be involved in development of the cervix. Mutations in the mouse gene lead to neural tube def

Entrez Gene ID

8092

UniProt ID

Q15699

Alternative Names

ALX Homeobox 1; Cartilage Paired-Class Homeoprotein 1; CART-1; CART1; Epididymis Luminal Protein 23; Cartilage Homeoprotein 1; ALX Homeobox Protein 1; HEL23; FND3;

Function

Sequence-specific DNA-binding transcription factor that binds palindromic sequences within promoters and may activate or repress the transcription of a subset of genes (PubMed:9753625, PubMed:8756334). Most probably regulates the expression of genes involved in the development of mesenchyme-derived craniofacial structures. Early on in development, it plays a role in forebrain mesenchyme survival (PubMed:20451171). May also induce epithelial to mesenchymal transition (EMT) through the expression of SNAI1 (PubMed:23288509).

Biological Process

Embryonic skeletal system morphogenesis Source: GO_Central
Negative regulation of transcription, DNA-templated Source: GO_Central
Negative regulation of transcription by RNA polymerase II Source: MGI
Neural crest cell migration Source: InterPro
Positive regulation of epithelial to mesenchymal transition Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Involvement in disease

Frontonasal dysplasia 3 (FND3): The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline.

PTM

Acetylated at Lys-131 by EP300; increases interaction with EP300 and stimulates ALX1 transcriptional activity.

Xiao, X., Chen, Y., Peng, L., & Zhang, T. (2021). Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system. Stem Cell Research, 53, 102309.

Vargel, I., Canter, H. I., Kucukguven, A., Aydin, A., & Ozgur, F. (2021). ALX-Related Frontonasal Dysplasias: Clinical Characteristics and Surgical Management. The Cleft Palate-Craniofacial Journal, 10556656211019621.

Pini, J., Kueper, J., Hu, Y. D., Kawasaki, K., Yeung, P., Tsimbal, C., ... & Liao, E. C. (2020). ALX 1‐related frontonasal dysplasia results from defective neural crest cell development and migration. EMBO molecular medicine, 12(10), e12013.

Jiao, J. X., Jiao, L. J., Yang, S., & Zhao, Y. J. (2019). Knockdown of aristaless-like homeobox1 inhibits epithelial-mesenchymal transition through Wnt/β-catenin signaling pathway in melanoma cells. Biochemical and biophysical research communications, 511(1), 105-110.

PINI, J., & Liao, E. C. (2018). Requirement Of ALX1 Homeobox Transcription Factor In Craniofacial Development. Plastic and Reconstructive Surgery Global Open.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ALX1 Recombinant Antibody (2A10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-ALX1 Recombinant Antibody (96k) (CAT#: CBMAB-C0616-FY)

Mouse Anti-ALX1 Recombinant Antibody (CBYY-C0953) (CAT#: CBMAB-C2390-YY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry