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Mouse Anti-ADORA2A Recombinant Antibody (CBT2923) (V2LY-0625-LY806)

Tested Data

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT2923
Antibody Isotype
IgG2a
Application
ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
ICC1:100-1:500
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adenosine A2a Receptor
Entrez Gene ID
UniProt ID
Function
Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Apoptotic process
Astrocyte activation
Blood circulation
Blood coagulation
Cell-cell signaling
Cellular defense response
Cellular protein metabolic process
Central nervous system development
Eating behavior
Excitatory postsynaptic potential
G protein-coupled receptor signaling pathway
Inflammatory response
Locomotory behavior
Membrane depolarization
Negative regulation of cell population proliferation
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
Negative regulation of inflammatory response to antigenic stimulus
Negative regulation of locomotion
Negative regulation of neuron apoptotic process
Negative regulation of protein kinase activity
Negative regulation of vascular permeability
Neuron projection morphogenesis
Phagocytosis
Positive regulation of acetylcholine secretion, neurotransmission
Positive regulation of circadian sleep/wake cycle, sleep
Positive regulation of glutamate secretion
Positive regulation of long-term synaptic potentiation
Positive regulation of protein secretion
Positive regulation of renal sodium excretion
Positive regulation of synaptic transmission, glutamatergic
Positive regulation of urine volume
Prepulse inhibition
Protein kinase C-activating G protein-coupled receptor signaling pathway
Regulation of calcium ion transport
Regulation of mitochondrial membrane potential
Regulation of norepinephrine secretion
Regulation of synaptic vesicle exocytosis
Regulation of transcription, DNA-templated
Response to caffeine
Response to drug
Sensory perception
Synaptic transmission, cholinergic
Synaptic transmission, glutamatergic
Vasodilation
Cellular Location
Cell membrane. Colocalizes with GAS2L2 at neuronal processes.
Topology
Extracellular: 1-7 aa
Helical: 8-32 aa
Cytoplasmic: 33-42 aa
Helical: 43-66 aa
Extracellular: 67-77 aa
Helical: 78-100 aa
Cytoplasmic: 101-120 aa
Helical: 121-143 aa
Extracellular: 144-173 aa
Helical: 174-198 aa
Cytoplasmic: 199-234 aa
Helical: 235-258 aa
Extracellular: 259-266 aa
Helical: 267-290 aa
Cytoplasmic: 291-412 aa
PTM
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
More Infomation

Newton, H. S., Chimote, A. A., Arnold, M. J., Wise-Draper, T. M., & Conforti, L. (2021). Targeted knockdown of the adenosine A2A receptor by lipid NPs rescues the chemotaxis of head and neck cancer memory T cells. Molecular Therapy-Methods & Clinical Development, 21, 133-143.

Akhtari, M., Vojdanian, M., Javinani, A., Ashraf-Ganjouei, A., Jamshidi, A., & Mahmoudi, M. (2020). Activation of adenosine A2A receptor induced interleukin-23 mRNA expression in macrophages of ankylosing spondylitis patients. Cytokine, 128, 154997.

Nardin, M., Verdoia, M., Negro, F., Suryapranata, H., Khedi, E., & De Luca, G. (2020). Relationship between adenosine A2a receptor polymorphism rs5751876 and fractional flow reserve during percutaneous coronary intervention. Heart and vessels, 35(10), 1349-1359.

Zhou, Y., Zeng, X., Li, G., Yang, Q., Xu, J., Zhang, M., ... & Huo, Y. (2019). Inactivation of endothelial adenosine A2A receptors protects mice from cerebral ischaemia‐induced brain injury. British journal of pharmacology, 176(13), 2250-2263.

Allard, B., Cousineau, I., Allard, D., Buisseret, L., Pommey, S., Chrobak, P., & Stagg, J. (2019). Adenosine A2a receptor promotes lymphangiogenesis and lymph node metastasis. Oncoimmunology, 8(8), 1601481.

Nardin, M., Verdoia, M., Pergolini, P., Rolla, R., Barbieri, L., Marino, P., ... & Novara Atherosclerosis Study Group. (2018). Impact of adenosine A2a receptor polymorphism rs5751876 on platelet reactivity in ticagrelor treated patients. Pharmacological research, 129, 27-33.

Vogt, T. J., Gevensleben, H., Dietrich, J., Kristiansen, G., Bootz, F., Landsberg, J., ... & Dietrich, D. (2018). Detailed analysis of adenosine A2a receptor (ADORA2A) and CD73 (5′-nucleotidase, ecto, NT5E) methylation and gene expression in head and neck squamous cell carcinoma patients. Oncoimmunology, 7(8), e1452579.

O'Connor, P. J., Loy, B. D., Lindheimer, J. B., & Covert, S. F. (2018). Adenosine A2A receptor gene polymorphisms (ADORA2A) are associated with maximal concentric contraction pain. Meta Gene, 18, 53-57.

Liu, Z., Yan, S., Wang, J., Xu, Y., Wang, Y., Zhang, S., ... & Huo, Y. (2017). Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis. Nature communications, 8(1), 1-18.

Nishiyama, K., Suzuki, H., Maruyama, M., Yoshihara, T., & Ohta, H. (2017). Genetic deletion of GPR52 enhances the locomotor-stimulating effect of an adenosine A2A receptor antagonist in mice: a potential role of GPR52 in the function of striatopallidal neurons. Brain research, 1670, 24-31.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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