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Mouse Anti-ADORA2A Recombinant Antibody (V2-180009) (CBMAB-A1396-YC)

Provided herein is a Mouse monoclonal antibody against Human Adenosine A2a Receptor. The antibody can be used for immunoassay techniques, such as WB, FC, ICC, IF, IHC-P, IHC-Fr.
See all ADORA2A antibodies
Published Data
Mouse Anti-ADORA2A Recombinant Antibody (7F6-G5-A2) (CBMAB-A1396-YC) in FC
CD4+ T cells were stimulated for 0-7 days with anti-CD3 and CD28 antibodies (Abs) in the presence of adenosine (0-1 mM). After stimulation, cells were stained with a FITC-conjugated anti-mouse CD4 Ab (x-axis) and a PE-conjugated anti-A2aR Ab (y-axis). First row: Splenocytes (left) or isolated CD4+ T cells (right) were stained with anti-CD4 (x-axis) and A2aR (y-axis) Abs. Second row: CD3/CD28-stimulated CD4+ T cells were stained with anti-CD4 (x-axis) and A2aR (y-axis) Abs. Third row: CD4+ T cells were stimulated with anti-CD3 and CD28 Abs in the presence of adenosine (0-1 mM) and then stained with anti-CD4 (x-axis) and A2aR (y-axis) Abs. The number in each panel represents the percentage ± SD of A2aRhigh cells within the CD4+ population (n = 3). Data were compared using one-way ANOVA with Tukey's post-hoc test. *P < 0.05 and **P < 0.01, compared with CD3/CD28 stimulation (second and third rows). ...View More
Tokano, M., Matsushita, S., Takagi, R., Yamamoto, T., & Kawano, M. (2022). Extracellular adenosine induces hypersecretion of IL-17A by T-helper 17 cells through the adenosine A2a receptor. Brain, Behavior, & Immunity-Health, 26, 100544.
Mouse Anti-ADORA2A Recombinant Antibody (7F6-G5-A2) (CBMAB-A1396-YC) in Immunoblot
Infected RAW 264.7 cells were pre-treated either with ZM241385 or MRS1754 and the expression of A2AR and A2BR were measured at protein level by immunoblotting. ...View More
Basu, M., Gupta, P., Dutta, A., Jana, K., & Ukil, A. (2020). Increased host ATP efflux and its conversion to extracellular adenosine is crucial for establishing Leishmania infection. Journal of cell science, 133(7), jcs239939.
Mouse Anti-ADORA2A Recombinant Antibody (7F6-G5-A2) (CBMAB-A1396-YC) in IHC
Distribution of adenosine receptor subtypes within the fetal left ventricle. Immunohistochemistry of adenosine receptors shown at low magnification (×20 objective, scale bar 100 μm) and higher magnification (×40 objective, scale bar 10 μm). Adenosine receptors are labeled in green, whereas the endothelium is labeled red, and nuclei are labeled in blue. In the low magnification image, a large vessel, connective tissue, and myocardium are labeled. The line trace (yellow arrow) crosses a myocyte and small vessel in the ×40 image, and the intensity of staining along the trace line is plotted in the graphs. ...View More
Davis, L., Musso, J., Soman, D., Louey, S., Nelson, J. W., & Jonker, S. S. (2018). Role of adenosine signaling in coordinating cardiomyocyte function and coronary vascular growth in chronic fetal anemia. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 315(3), R500-R508.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Pig, Dog, Guinea pig, Rabbit
Clone
V2-180009
Antibody Isotype
IgG2a
Application
WB, IHC-F, ICC, IF, IHC-P

Basic Information

Immunogen
Full length recombinant human Adenosine A2a R.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Pig, Dog, Guinea pig, Rabbit
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adenosine A2a Receptor
Introduction
ADORA2A is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activa
Entrez Gene ID
Human135
Mouse11540
Rat25369
Pig503661
Dog403960
Guineapig
Rabbit100339944
UniProt ID
HumanP29274
MouseQ60613
RatP30543
PigF1RLS0
DogP11617
Guineapig
RabbitG1SVQ2
Alternative Names
Adenosine A2a Receptor; ADORA2; Adenosine Receptor Subtype A2a; Adenosine Receptor A2a; A2aR; RDC8;
Function
Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Apoptotic process
Astrocyte activation
Blood circulation
Blood coagulation
Cell-cell signaling
Cellular defense response
Cellular protein metabolic process
Central nervous system development
Eating behavior
Excitatory postsynaptic potential
G protein-coupled receptor signaling pathway
Inflammatory response
Locomotory behavior
Membrane depolarization
Negative regulation of cell population proliferation
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
Negative regulation of inflammatory response to antigenic stimulus
Negative regulation of locomotion
Negative regulation of neuron apoptotic process
Negative regulation of protein kinase activity
Negative regulation of vascular permeability
Neuron projection morphogenesis
Phagocytosis
Positive regulation of acetylcholine secretion, neurotransmission
Positive regulation of circadian sleep/wake cycle, sleep
Positive regulation of glutamate secretion
Positive regulation of long-term synaptic potentiation
Positive regulation of protein secretion
Positive regulation of renal sodium excretion
Positive regulation of synaptic transmission, glutamatergic
Positive regulation of urine volume
Prepulse inhibition
Protein kinase C-activating G protein-coupled receptor signaling pathway
Regulation of calcium ion transport
Regulation of mitochondrial membrane potential
Regulation of norepinephrine secretion
Regulation of synaptic vesicle exocytosis
Regulation of transcription, DNA-templated
Response to caffeine
Response to drug
Sensory perception
Synaptic transmission, cholinergic
Synaptic transmission, glutamatergic
Vasodilation
Cellular Location
Cell membrane. Colocalizes with GAS2L2 at neuronal processes.
Topology
Extracellular: 1-7 aa
Helical: 8-32 aa
Cytoplasmic: 33-42 aa
Helical: 43-66 aa
Extracellular: 67-77 aa
Helical: 78-100 aa
Cytoplasmic: 101-120 aa
Helical: 121-143 aa
Extracellular: 144-173 aa
Helical: 174-198 aa
Cytoplasmic: 199-234 aa
Helical: 235-258 aa
Extracellular: 259-266 aa
Helical: 267-290 aa
Cytoplasmic: 291-412 aa
PTM
Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.

Newton, H. S., Chimote, A. A., Arnold, M. J., Wise-Draper, T. M., & Conforti, L. (2021). Targeted knockdown of the adenosine A2A receptor by lipid NPs rescues the chemotaxis of head and neck cancer memory T cells. Molecular Therapy-Methods & Clinical Development, 21, 133-143.

Akhtari, M., Vojdanian, M., Javinani, A., Ashraf-Ganjouei, A., Jamshidi, A., & Mahmoudi, M. (2020). Activation of adenosine A2A receptor induced interleukin-23 mRNA expression in macrophages of ankylosing spondylitis patients. Cytokine, 128, 154997.

Nardin, M., Verdoia, M., Negro, F., Suryapranata, H., Khedi, E., & De Luca, G. (2020). Relationship between adenosine A2a receptor polymorphism rs5751876 and fractional flow reserve during percutaneous coronary intervention. Heart and vessels, 35(10), 1349-1359.

Zhou, Y., Zeng, X., Li, G., Yang, Q., Xu, J., Zhang, M., ... & Huo, Y. (2019). Inactivation of endothelial adenosine A2A receptors protects mice from cerebral ischaemia‐induced brain injury. British journal of pharmacology, 176(13), 2250-2263.

Allard, B., Cousineau, I., Allard, D., Buisseret, L., Pommey, S., Chrobak, P., & Stagg, J. (2019). Adenosine A2a receptor promotes lymphangiogenesis and lymph node metastasis. Oncoimmunology, 8(8), 1601481.

Nardin, M., Verdoia, M., Pergolini, P., Rolla, R., Barbieri, L., Marino, P., ... & Novara Atherosclerosis Study Group. (2018). Impact of adenosine A2a receptor polymorphism rs5751876 on platelet reactivity in ticagrelor treated patients. Pharmacological research, 129, 27-33.

Vogt, T. J., Gevensleben, H., Dietrich, J., Kristiansen, G., Bootz, F., Landsberg, J., ... & Dietrich, D. (2018). Detailed analysis of adenosine A2a receptor (ADORA2A) and CD73 (5′-nucleotidase, ecto, NT5E) methylation and gene expression in head and neck squamous cell carcinoma patients. Oncoimmunology, 7(8), e1452579.

O'Connor, P. J., Loy, B. D., Lindheimer, J. B., & Covert, S. F. (2018). Adenosine A2A receptor gene polymorphisms (ADORA2A) are associated with maximal concentric contraction pain. Meta Gene, 18, 53-57.

Liu, Z., Yan, S., Wang, J., Xu, Y., Wang, Y., Zhang, S., ... & Huo, Y. (2017). Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis. Nature communications, 8(1), 1-18.

Nishiyama, K., Suzuki, H., Maruyama, M., Yoshihara, T., & Ohta, H. (2017). Genetic deletion of GPR52 enhances the locomotor-stimulating effect of an adenosine A2A receptor antagonist in mice: a potential role of GPR52 in the function of striatopallidal neurons. Brain research, 1670, 24-31.

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For research use only. Not intended for any clinical use.

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