Mouse Anti-ADORA2A Recombinant Antibody (V2-180012) (CBMAB-A1399-YC)

Name: Mouse Anti-ADORA2A Recombinant Antibody (V2-180012)
SKU: CBMAB-A1399-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ADORA2A Recombinant Antibody (CBYC-A377) (CBMAB-A1399-YC) in Immunoblot

A2A-R down regulation under t-BHP treatment. 4 pmol of siRNA A2A-R were added to primary cultures of neurons and 24h later t-BHP was added to cells, during 18h; lane 1, neurons under normal conditions, lane 2, neurons+siA2A-R under 0 μM t-BHP, lane 3, neurons+siA2A-R under 20 μM tBHP, lane 4, neurons+siA2A-R under 40 μM t-BHP. This experiment was repeated three times with similar results. ...View More

Queiroga, C. S., Alves, R. M., Conde, S. V., Alves, P. M., & Vieira, H. L. (2016). Paracrine effect of carbon monoxide-astrocytes promote neuroprotection through purinergic signaling in mice. Journal of Cell Science, 129(16), 3178-3188.

Mouse Anti-ADORA2A Recombinant Antibody (CBYC-A377) (CBMAB-A1399-YC) in ICC

Representative single nerve terminal immunocytochemistry identifying that vesicular glutamate transporter type 1 (vGluT1, a marker of glutamatergic terminals; green) and mCherry immunoreactivity (red) were found to be co-localized (arrows identifying yellow in 'merged') in hippocampal synaptosomes from mice transfected with AAV-mCherry-optoA2AR (optoA2AR), whereas this was not observed for mice transfected with AAV-mCherry (not shown) (n=3). ...View More

Li, P., Rial, D., Canas, P. M., Yoo, J. H., Li, W., Zhou, X., ... & Chen, J. F. (2015). Optogenetic activation of intracellular adenosine A2A receptor signaling in the hippocampus is sufficient to trigger CREB phosphorylation and impair memory. Molecular psychiatry, 20(11), 1339-1349.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180012

Application

WB, IP, IF, ELISA

Immunogen

Raised against His-tagged full length human recombinant Adenosine A2A receptor, epitope mapping to the third intracellular loop.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, 50% glycerol, 0.05% azide

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adenosine A2a Receptor

Introduction

ADORA2A is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activa

Entrez Gene ID

135

UniProt ID

P29274

Alternative Names

Adenosine A2a Receptor; ADORA2; Adenosine Receptor Subtype A2a; Adenosine Receptor A2a; A2aR; RDC8;

Function

Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Apoptotic process
Astrocyte activation
Blood circulation
Blood coagulation
Cell-cell signaling
Cellular defense response
Cellular protein metabolic process
Central nervous system development
Eating behavior
Excitatory postsynaptic potential
G protein-coupled receptor signaling pathway
Inflammatory response
Locomotory behavior
Membrane depolarization
Negative regulation of cell population proliferation
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
Negative regulation of inflammatory response to antigenic stimulus
Negative regulation of locomotion
Negative regulation of neuron apoptotic process
Negative regulation of protein kinase activity
Negative regulation of vascular permeability
Neuron projection morphogenesis
Phagocytosis
Positive regulation of acetylcholine secretion, neurotransmission
Positive regulation of circadian sleep/wake cycle, sleep
Positive regulation of glutamate secretion
Positive regulation of long-term synaptic potentiation
Positive regulation of protein secretion
Positive regulation of renal sodium excretion
Positive regulation of synaptic transmission, glutamatergic
Positive regulation of urine volume
Prepulse inhibition
Protein kinase C-activating G protein-coupled receptor signaling pathway
Regulation of calcium ion transport
Regulation of mitochondrial membrane potential
Regulation of norepinephrine secretion
Regulation of synaptic vesicle exocytosis
Regulation of transcription, DNA-templated
Response to caffeine
Response to drug
Sensory perception
Synaptic transmission, cholinergic
Synaptic transmission, glutamatergic
Vasodilation

Cellular Location

Cell membrane. Colocalizes with GAS2L2 at neuronal processes.

Topology

Extracellular: 1-7 aa
Helical: 8-32 aa
Cytoplasmic: 33-42 aa
Helical: 43-66 aa
Extracellular: 67-77 aa
Helical: 78-100 aa
Cytoplasmic: 101-120 aa
Helical: 121-143 aa
Extracellular: 144-173 aa
Helical: 174-198 aa
Cytoplasmic: 199-234 aa
Helical: 235-258 aa
Extracellular: 259-266 aa
Helical: 267-290 aa
Cytoplasmic: 291-412 aa

PTM

Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.

Newton, H. S., Chimote, A. A., Arnold, M. J., Wise-Draper, T. M., & Conforti, L. (2021). Targeted knockdown of the adenosine A2A receptor by lipid NPs rescues the chemotaxis of head and neck cancer memory T cells. Molecular Therapy-Methods & Clinical Development, 21, 133-143.

Akhtari, M., Vojdanian, M., Javinani, A., Ashraf-Ganjouei, A., Jamshidi, A., & Mahmoudi, M. (2020). Activation of adenosine A2A receptor induced interleukin-23 mRNA expression in macrophages of ankylosing spondylitis patients. Cytokine, 128, 154997.

Nardin, M., Verdoia, M., Negro, F., Suryapranata, H., Khedi, E., & De Luca, G. (2020). Relationship between adenosine A2a receptor polymorphism rs5751876 and fractional flow reserve during percutaneous coronary intervention. Heart and vessels, 35(10), 1349-1359.

Zhou, Y., Zeng, X., Li, G., Yang, Q., Xu, J., Zhang, M., ... & Huo, Y. (2019). Inactivation of endothelial adenosine A2A receptors protects mice from cerebral ischaemia‐induced brain injury. British journal of pharmacology, 176(13), 2250-2263.

Allard, B., Cousineau, I., Allard, D., Buisseret, L., Pommey, S., Chrobak, P., & Stagg, J. (2019). Adenosine A2a receptor promotes lymphangiogenesis and lymph node metastasis. Oncoimmunology, 8(8), 1601481.

Nardin, M., Verdoia, M., Pergolini, P., Rolla, R., Barbieri, L., Marino, P., ... & Novara Atherosclerosis Study Group. (2018). Impact of adenosine A2a receptor polymorphism rs5751876 on platelet reactivity in ticagrelor treated patients. Pharmacological research, 129, 27-33.

Vogt, T. J., Gevensleben, H., Dietrich, J., Kristiansen, G., Bootz, F., Landsberg, J., ... & Dietrich, D. (2018). Detailed analysis of adenosine A2a receptor (ADORA2A) and CD73 (5′-nucleotidase, ecto, NT5E) methylation and gene expression in head and neck squamous cell carcinoma patients. Oncoimmunology, 7(8), e1452579.

O'Connor, P. J., Loy, B. D., Lindheimer, J. B., & Covert, S. F. (2018). Adenosine A2A receptor gene polymorphisms (ADORA2A) are associated with maximal concentric contraction pain. Meta Gene, 18, 53-57.

Liu, Z., Yan, S., Wang, J., Xu, Y., Wang, Y., Zhang, S., ... & Huo, Y. (2017). Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis. Nature communications, 8(1), 1-18.

Nishiyama, K., Suzuki, H., Maruyama, M., Yoshihara, T., & Ohta, H. (2017). Genetic deletion of GPR52 enhances the locomotor-stimulating effect of an adenosine A2A receptor antagonist in mice: a potential role of GPR52 in the function of striatopallidal neurons. Brain research, 1670, 24-31.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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