Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-COMT Recombinant Antibody (C2200) (V2LY-1206-LY743)


See all COMT antibodies
Online Inquiry
Published Data
V2LY-1206-LY743-1
Mouse Anti-COMT Recombinant Antibody (C2200) (V2LY-1206-LY743) in IF
Immunostaining of COMT in H9-dervied NE neurons at day 30. Scale bar, 20 µm. ...View More
Tao, Y., Li, X., Dong, Q., Kong, L., Petersen, A. J., Yan, Y., ... & Zhang, S. C. (2024). Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells. Nature Biotechnology, 42(9), 1404-1416.
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY743-2
Mouse Anti-COMT Recombinant Antibody (C2200) (V2LY-1206-LY743) in WB
Effects of the BDNF i.c.v. administration on protein levels of COMT in various brain regions of BTBR mice. ...View More
Ilchibaeva, T., Tsybko, A., Lipnitskaya, M., Eremin, D., Milutinovich, K., Naumenko, V., & Popova, N. (2023). Brain-derived neurotrophic factor (BDNF) in mechanisms of autistic-like behavior in BTBR mice: Crosstalk with the dopaminergic brain system. Biomedicines, 11(5), 1482.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human
Clone
C2200
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-271 representing full length catechol-O-methyltranferase of human.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
<0.1% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Catechol-O-Methyltransferase
Entrez Gene ID
1312
UniProt ID
P21964
Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Biological Process
Catecholamine catabolic process Source: UniProtKB
Developmental process Source: GO_Central
Dopamine metabolic process Source: GO_Central
Methylation Source: UniProtKB
Neurotransmitter catabolic process Source: UniProtKB-KW
Cellular Location
Isoform Soluble: Cytoplasm
Isoform Membrane-bound: Cell membrane
Involvement in disease
Schizophrenia (SCZD):
A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
Topology
Cytoplasmic: 1-6
Helical: 7-26
Extracellular: 27-271
PTM
The N-terminus is blocked.
More Infomation

Sprague, B. N., Rosso, A. L., Zhu, X., Bohnen, N. I., & Rosano, C. (2021). Catechol‐O‐methyltransferase (COMT) polymorphism predicts rapid gait speed changes in healthy older adults. Journal of the American Geriatrics Society, 69(11), 3194-3202.

Cao, C., Cao, L., & Chen, J. (2019). Differences in sensitivity to environment depending on Catechol-O-Methyltransferase (COMT) gene? A meta-analysis of child and adolescent gene-by-environment studies. Journal of Youth and Adolescence, 48(4), 655-667.

Nogueira, N. G., Fernandes, L. A., Ferreira, B. P., Batista, M. T., Alves, K. C., & Parma, J. O. (2019). Association between the Catechol-O-Methyltransferase (COMT) Val158Met polymorphism and manual performance asymmetries. Perceptual and Motor Skills, 126(3), 349-365.

Nogueira, N. G. D. H. M., Bacelar, M. F. B., de Paula Ferreira, B., Parma, J. O., & Lage, G. M. (2019). Association between the catechol-O-methyltransferase (COMT) Val158Met polymorphism and motor behavior in healthy adults: a study review. Brain Research Bulletin, 144, 223-232.

Satterfield, B. C., Hinson, J. M., Whitney, P., Schmidt, M. A., Wisor, J. P., & Van Dongen, H. P. (2018). Catechol-O-methyltransferase (COMT) genotype affects cognitive control during total sleep deprivation. Cortex, 99, 179-186.

Patel, C. N., Georrge, J. J., Modi, K. M., Narechania, M. B., Patel, D. P., Gonzalez, F. J., & Pandya, H. A. (2018). Pharmacophore-based virtual screening of catechol-o-methyltransferase (COMT) inhibitors to combat Alzheimer’s disease. Journal of Biomolecular Structure and Dynamics, 36(15), 3938-3957.

Parkin, G. M., Udawela, M., Gibbons, A., Scarr, E., & Dean, B. (2018). Catechol-O-methyltransferase (COMT) genotypes are associated with varying soluble, but not membrane-bound COMT protein in the human prefrontal cortex. Journal of human genetics, 63(12), 1251-1258.

Lin, C. H., Chaudhuri, K., Fan, J. Y., Ko, C. I., Rizos, A., Chang, C. W., ... & Wu, Y. R. (2017). Depression and Catechol-O-methyltransferase (COMT) genetic variants are associated with pain in Parkinson’s disease. Scientific reports, 7(1), 1-10.

Bastos, P., Gomes, T., & Ribeiro, L. (2017). Catechol-O-methyltransferase (COMT): an update on its role in cancer, neurological and cardiovascular diseases. Reviews of Physiology, Biochemistry and Pharmacology, Vol. 173, 1-39.

Matsuzaka, C. T., Christofolini, D., Ota, V. K., Gadelha, A., Berberian, A. A., Noto, C., ... & Bressan, R. A. (2017). Catechol-O-methyltransferase (COMT) polymorphisms modulate working memory in individuals with schizophrenia and healthy controls. Brazilian Journal of Psychiatry, 39, 302-308.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-COMT Recombinant Antibody (C2200)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry