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Mouse Anti-DCTN2 Recombinant Antibody (D0409) (CBMAB-D0409-YC)


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Published Data
CBMAB-D0409-YC-1
Mouse Anti-DCTN2 Recombinant Antibody (D0409) (CBMAB-D0409-YC) in WB
A representative SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of purified Dynein, Dynactin, and BICD2N (truncated to first 594 aa., untagged and tagged with an enhanced green fluorescent protein (eGFP)) and Kinesin-3 (KIF16B, untagged and tagged with eGFP). Identity of DHC, IC2, p150, p62, p50, and Arp1 were confirmed with Western blotting using appropriate antibodies (see Methods). All experiments described in the paper were performed with at least two independent protein preparations. ...View More
D'Souza, A. I., Grover, R., Monzon, G. A., Santen, L., & Diez, S. (2023). Vesicles driven by dynein and kinesin exhibit directional reversals without regulators. Nature communications, 14(1), 7532. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-D0409-YC-2
Mouse Anti-DCTN2 Recombinant Antibody (D0409) (CBMAB-D0409-YC) in WB
DCNT2 is a direct FER substrate. Shown are kinase assays using FER-D742R and FER-AS in the presence of ATPγS, using recombinant GST-DCTN2 as substrate. GST-FER served as loading control. ...View More
Tavares, S., Liv, N., Pasolli, M., Opdam, M., Rätze, M. A., Saornil, M., ... & Derksen, P. W. (2022). FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancer. Cell Reports, 39(1). Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-D0409-YC-3
Mouse Anti-DCTN2 Recombinant Antibody (D0409) (CBMAB-D0409-YC) in IF
DCNT2 is essential for BC cells motility. DCTN2 was knocked down in cells were stained for DCTN2 (cyan). Scale bars, 50 μm. ...View More
Tavares, S., Liv, N., Pasolli, M., Opdam, M., Rätze, M. A., Saornil, M., ... & Derksen, P. W. (2022). FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancer. Cell Reports, 39(1). Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D0409
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 102-401 mapping at the C-terminus of human Dynactin 2.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml

Target

Full Name
Dynactin Subunit 2
Entrez Gene ID
Human10540
Mouse69654
Rat299850
UniProt ID
HumanQ13561
MouseQ99KJ8
RatQ6AYH5
Function
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Ciliary basal body-plasma membrane docking Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Melanosome transport Source: Ensembl
Mitotic metaphase plate congression Source: UniProtKB
Mitotic spindle organization Source: UniProtKB
Protein localization to centrosome Source: SYSCILIA_CCNET
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Cellular Location
Centrosome; Membrane
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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