Mouse Anti-DCTN2 Recombinant Antibody (770) (V2LY-0125-LY1250)

Name: Mouse Anti-DCTN2 Recombinant Antibody (770)
SKU: V2LY-0125-LY1250
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

770

Antibody Isotype

IgG2b

Application

WB, IF

Basic Information

Immunogen

E. coli-derived recombinant human Dynactin Subunit 2/DCTN2, Asp277-Lys401.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	2 μg/mL
IF(ICC)	8-25 μg/mL

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS & Trehalose

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

10540

UniProt ID

Q13561

Function

Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. Involved in anchoring microtubules to centrosomes. May play a role in synapse formation during brain development.

Biological Process

Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
Ciliary basal body-plasma membrane docking Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Melanosome transport Source: Ensembl
Mitotic metaphase plate congression Source: UniProtKB
Mitotic spindle organization Source: UniProtKB
Protein localization to centrosome Source: SYSCILIA_CCNET
Regulation of G2/M transition of mitotic cell cycle Source: Reactome

Cellular Location

Centrosome; Membrane

More Infomation

References

Li, Y. F., Shi, L. J., Wang, P., Wang, J. W., Shi, G. Y., & Lee, S. C. (2021). Binding between ROCK1 and DCTN2 triggers diabetes‑associated centrosome amplification in colon cancer cells. Oncology reports, 46(1), 1-11.

Lee, Y. D., Kim, B., Jung, S., Kim, H., Kim, M. K., Kwon, J. O., ... & Kim, H. H. (2020). The dynactin subunit DCTN1 controls osteoclastogenesis via the Cdc42/PAK2 pathway. Experimental & molecular medicine, 52(3), 514-528.

D’Aurora, M., Budani, M. C., Franchi, S., Sarra, A., Stuppia, L., Tiboni, G. M., & Gatta, V. (2019). Dynactin pathway-related gene expression is altered by aging, but not by vitrification. Reproductive Toxicology, 88, 48-55.

Wang, S., Wang, Q., Zhang, X., Liao, X., Wang, G., Yu, L., ... & Yuan, W. (2018). Distinct prognostic value of dynactin subunit 4 (DCTN4) and diagnostic value of DCTN1, DCTN2, and DCTN4 in colon adenocarcinoma. Cancer Management and Research, 10, 5807.

Shen, C., Honda, H., Suzuki, S. O., Maeda, N., Shijo, M., Hamasaki, H., ... & Iwaki, T. (2018). Dynactin is involved in Lewy body pathology. Neuropathology, 38(6), 583-590.

Wang, Q., Wang, X., Liang, Q., Wang, S., Liao, X., Li, D., & Pan, F. (2018). Prognostic value of dynactin mRNA expression in cutaneous melanoma. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 24, 3752.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

DCTN2 Matched Antibody Pair (325) (CAT#: APMAB-325LY)

Related Products

Mouse Anti-DCTN2 Recombinant Antibody (D0409) (CAT#: CBMAB-D0409-YC)

Mouse Anti-DCTN2 Recombinant Antibody (2G7) (CAT#: V2LY-0125-LY1248)

DCTN2 Matched Antibody Pair (325) (CAT#: APMAB-325LY)

Mouse Anti-DCTN2 Recombinant Antibody (AP1833) (CAT#: V2LY-0125-LY1251)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
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Second antibody and isotype control

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